This assay has high sensitivity and excellent specificity for detection of Electron Transfer Flavoprotein Beta Polypeptide (ETFb). No significant cross-reactivity or interference between Electron Transfer Flavoprotein Beta Polypeptide (ETFb) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Reverse Triiodothyronine (rT3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Reverse Triiodothyronine (rT3) and unlabeled High Sensitive Reverse Triiodothyronine (rT3) (Standards or samples) with the pre-coated antibody specific to High Sensitive Reverse Triiodothyronine (rT3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Reverse Triiodothyronine (rT3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Reverse Triiodothyronine (rT3) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人電子轉移黃素蛋白β多肽(ETFb)ELISA試劑盒產品,Short term: 4°C; Long term: see manual.