This assay has high sensitivity and excellent specificity for detection of Engrailed Homeobox Protein 2 (EN2). No significant cross-reactivity or interference between Engrailed Homeobox Protein 2 (EN2) and analogues was observed.
產品描述
The microplate provided in this kit has been pre-coated with an antibody specific to S100A8. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to S100A9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain calprotectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人Engrailed同源盒蛋白2(EN2)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
