This assay has high sensitivity and excellent specificity for detection of Eosinophil Chemotactic Factor (ECF). No significant cross-reactivity or interference between Eosinophil Chemotactic Factor (ECF) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Folic Acid (FA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Folic Acid (FA) and unlabeled Folic Acid (FA) (Standards or samples) with the pre-coated antibody specific to Folic Acid (FA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Folic Acid (FA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Folic Acid (FA) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該小鼠嗜酸性粒細胞趨化因子(ECF)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
