This assay has high sensitivity and excellent specificity for detection of Erythropoietin (EPO). No significant cross-reactivity or interference between Erythropoietin (EPO) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Prostaglandin H2 (PGH2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Prostaglandin H2 (PGH2) and unlabeled Prostaglandin H2 (PGH2) (Standards or samples) with the pre-coated antibody specific to Prostaglandin H2 (PGH2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Prostaglandin H2 (PGH2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Prostaglandin H2 (PGH2) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該小鼠促紅細胞生成素(EPO)ELISA試劑盒產品,Short term: 4°C; Long term: see manual.