This assay has high sensitivity and excellent specificity for detection of Estradiol (E2). No significant cross-reactivity or interference between Estradiol (E2) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Competitive Inhibition
保存建議
Biomatik品牌廠家建議該雌二醇(E2)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
