This assay has high sensitivity and excellent specificity for detection of Extracellular Matrix Protein 1 (ECM1). No significant cross-reactivity or interference between Extracellular Matrix Protein 1 (ECM1) and analogues was observed.
產品描述
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Platelet Derived Growth Factor C (PDGFC). A competitive inhibition reaction is launched between biotin labeled Platelet Derived Growth Factor C (PDGFC) and unlabeled Platelet Derived Growth Factor C (PDGFC) (Standards or samples) with the pre-coated antibody specific to Platelet Derived Growth Factor C (PDGFC). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Platelet Derived Growth Factor C (PDGFC) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Platelet Derived Growth Factor C (PDGFC) level in the sample or standard.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人細胞外基質蛋白1(ECM1)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
