This assay has high sensitivity and excellent specificity for detection of Four And A Half LIM Domains Protein 1 (FHL1). No significant cross-reactivity or interference between Four And A Half LIM Domains Protein 1 (FHL1) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Oxidized Glutathione (GSSG) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Oxidized Glutathione (GSSG) and unlabeled Oxidized Glutathione (GSSG) (Standards or samples) with the pre-coated antibody specific to Oxidized Glutathione (GSSG). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Oxidized Glutathione (GSSG) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Oxidized Glutathione (GSSG) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人四個半LIM結構域蛋白1(FHL1)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
