This assay has high sensitivity and excellent specificity for detection of Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A). No significant cross-reactivity or interference between Inositol Polyphosphate-4-Phosphatase Type I 107kDa (INPP4A) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT Inhibitory Factor 1 (WIF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT Inhibitory Factor 1 (WIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT Inhibitory Factor 1 (WIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of WNT Inhibitory Factor 1 (WIF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該小鼠肌醇多磷酸-4-磷酸酶I型107kDa(INPP4A)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
