This assay has high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein, Acid Labile Subunit (IGFALS) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Electron Transfer Flavoprotein Alpha Polypeptide (ETFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Electron Transfer Flavoprotein Alpha Polypeptide (ETFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Electron Transfer Flavoprotein Alpha Polypeptide (ETFa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人胰島素樣生長因子結合蛋白，酸不穩定亞基(IGFALS)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
