This assay has high sensitivity and excellent specificity for detection of N-Ethylmaleimide Sensitive Factor (NSF). No significant cross-reactivity or interference between N-Ethylmaleimide Sensitive Factor (NSF) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Corticotropin Like Intermediate Lobe Peptide (CLIP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Corticotropin Like Intermediate Lobe Peptide (CLIP) and unlabeled Corticotropin Like Intermediate Lobe Peptide (CLIP) (Standards or samples) with the pre-coated antibody specific to Corticotropin Like Intermediate Lobe Peptide (CLIP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Corticotropin Like Intermediate Lobe Peptide (CLIP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Corticotropin Like Intermediate Lobe Peptide (CLIP) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人N-乙基馬來酰亞胺敏感因子(NSF)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
