This assay has high sensitivity and excellent specificity for detection of Parkinson Disease Protein 7 (PARK7). No significant cross-reactivity or interference between Parkinson Disease Protein 7 (PARK7) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD5 Antigen Like Protein (CD5L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CD5 Antigen Like Protein (CD5L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD5 Antigen Like Protein (CD5L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD5 Antigen Like Protein (CD5L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人類帕金森病蛋白7(PARK7)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
