This assay has high sensitivity and excellent specificity for detection of Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL). No significant cross-reactivity or interference between Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Osteogenic Growth Peptide (OGP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Osteogenic Growth Peptide (OGP) and unlabeled Osteogenic Growth Peptide (OGP) (Standards or samples) with the pre-coated antibody specific to Osteogenic Growth Peptide (OGP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Osteogenic Growth Peptide (OGP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Osteogenic Growth Peptide (OGP) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該小鼠核因子Kappa B配體受體激活劑(RANkL)ELISA試劑盒產品,Short term: 4°C; Long term: see manual.