This assay has high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 1 Alpha (REG1a). No significant cross-reactivity or interference between Regenerating Islet Derived Protein 1 Alpha (REG1a) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class II DM Beta (MHCDMb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class II DM Beta (MHCDMb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class II DM Beta (MHCDMb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class II DM Beta (MHCDMb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該小鼠再生胰島衍生蛋白1Alpha(REG1a)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
