This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A12 (S100A12). No significant cross-reactivity or interference between S100 Calcium Binding Protein A12 (S100A12) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Immunoglobulin G2 (IgG2) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Immunoglobulin G2 (IgG2) and unlabeled Immunoglobulin G2 (IgG2) (Standards or samples) with the pre-coated antibody specific to Immunoglobulin G2 (IgG2). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Immunoglobulin G2 (IgG2) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Immunoglobulin G2 (IgG2) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人S100鈣結合蛋白A12(S100A12)ELISA試劑盒產品,Short term: 4°C; Long term: see manual.