This assay has high sensitivity and excellent specificity for detection of Sulfatase Modifying Factor 1 (SUMF1). No significant cross-reactivity or interference between Sulfatase Modifying Factor 1 (SUMF1) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ghrelin/Obestatin Preprohormone (GHRL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ghrelin/Obestatin Preprohormone (GHRL) and unlabeled Ghrelin/Obestatin Preprohormone (GHRL) (Standards or samples) with the pre-coated antibody specific to Ghrelin/Obestatin Preprohormone (GHRL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ghrelin/Obestatin Preprohormone (GHRL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ghrelin/Obestatin Preprohormone (GHRL) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人硫酸酯酶修飾因子1(SUMF1)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
