This assay has high sensitivity and excellent specificity for detection of Suppressors Of Cytokine Signaling 3 (SOCS3). No significant cross-reactivity or interference between Suppressors Of Cytokine Signaling 3 (SOCS3) and analogues was observed.
產品描述
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Inositol Triphosphate (IP3). A competitive inhibition reaction is launched between biotin labeled Inositol Triphosphate (IP3) and unlabeled Inositol Triphosphate (IP3) (Standards or samples) with the pre-coated antibody specific to Inositol Triphosphate (IP3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inositol Triphosphate (IP3) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Inositol Triphosphate (IP3) level in the sample or standard.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人類細胞因子信號通路抑制劑3(SOCS3)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
