This assay has high sensitivity and excellent specificity for detection of Tissue Inhibitors Of Metalloproteinase 3 (TIMP3). No significant cross-reactivity or interference between Tissue Inhibitors Of Metalloproteinase 3 (TIMP3) and analogues was observed.
產品描述
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to 5-Hydroxytryptamine (5-HT). A competitive inhibition reaction is launched between biotin labeled 5-Hydroxytryptamine (5-HT) and unlabeled 5-Hydroxytryptamine (5-HT) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxytryptamine (5-HT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxytryptamine (5-HT) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the 5-Hydroxytryptamine (5-HT) level in the sample or standard.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該Simian Tissue Inhibitors Of Metalloproteinase3(TIMP3)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
