This assay has high sensitivity and excellent specificity for detection of Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1). No significant cross-reactivity or interference between Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neuromedin B (NMB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neuromedin B (NMB) and unlabeled Neuromedin B (NMB) (Standards or samples) with the pre-coated antibody specific to Neuromedin B (NMB). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neuromedin B (NMB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neuromedin B (NMB) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該具有免疫球蛋白樣和EGF樣結構域蛋白1(Tie1)ELISA試劑盒的小鼠酪氨酸激酶產品,Short term: 4°C; Long term: see manual.