This assay has high sensitivity and excellent specificity for detection of Chemokine Like Factor Superfamily 6 (CKLFSF6). No significant cross-reactivity or interference between Chemokine Like Factor Superfamily 6 (CKLFSF6) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 1 (LAMA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Laminin Alpha 1 (LAMA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Alpha 1 (LAMA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Alpha 1 (LAMA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人類趨化因子樣因子超家族6(CKLFSF6)CLIA試劑盒產品，Short term: 4°C; Long term: see manual.
