This assay has high sensitivity and excellent specificity for detection of NLR Family, Pyrin Domain Containing Protein 3 (NLRP3). No significant cross-reactivity or interference between NLR Family, Pyrin Domain Containing Protein 3 (NLRP3) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Hydroxytryptophan (5-HTP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Hydroxytryptophan (5-HTP) and unlabeled 5-Hydroxytryptophan (5-HTP) (Standards or samples) with the pre-coated antibody specific to 5-Hydroxytryptophan (5-HTP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Hydroxytryptophan (5-HTP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Hydroxytryptophan (5-HTP) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該大鼠NLR家族，含Pyrin結構域蛋白3(NLRP3)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
