This assay has high sensitivity and excellent specificity for detection of Wide-range Carnitine Palmitoyltransferase 1A, Liver (CPT1A). No significant cross-reactivity or interference between Wide-range Carnitine Palmitoyltransferase 1A, Liver (CPT1A) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and unlabeled Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) (Standards or samples) with the pre-coated antibody specific to Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人肉堿棕櫚酰轉移酶1A，肝臟(CPT1A)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
