This assay has high sensitivity and excellent specificity for detection of Alpha-1-Microglobulin (a1M). No significant cross-reactivity or interference between Alpha-1-Microglobulin (a1M) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Fibroblast Growth Factor 1, Acidic (FGF1) and unlabeled Fibroblast Growth Factor 1, Acidic (FGF1) (Standards or samples) with the pre-coated antibody specific to Fibroblast Growth Factor 1, Acidic (FGF1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Fibroblast Growth Factor 1, Acidic (FGF1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Fibroblast Growth Factor 1, Acidic (FGF1) in the sample.
產品特點
Competitive Inhibition
保存建議
Biomatik品牌廠家建議該小鼠Alpha-1-微球蛋白(a1M)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
