This assay has high sensitivity and excellent specificity for detection of Ectonucleoside Triphosphate Diphosphohydrolase 2 (ENTPD2). No significant cross-reactivity or interference between Ectonucleoside Triphosphate Diphosphohydrolase 2 (ENTPD2) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Slingshot Homolog 1 (SSH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Slingshot Homolog 1 (SSH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Slingshot Homolog 1 (SSH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Slingshot Homolog 1 (SSH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人外核苷三磷酸二磷酸水解酶2(ENTPD2)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
