This assay has high sensitivity and excellent specificity for detection of Elongation Of Very Long Chain Fatty Acids Like Protein 5 (ELOVL5). No significant cross-reactivity or interference between Elongation Of Very Long Chain Fatty Acids Like Protein 5 (ELOVL5) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人超長鏈脂肪酸如蛋白5(ELOVL5)ELISA試劑盒的延伸產品，Short term: 4°C; Long term: see manual.
