This assay has high sensitivity and excellent specificity for detection of SMAD family member 3 (SMAD3). No significant cross-reactivity or interference between SMAD family member 3 (SMAD3) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 3-Mercaptopyruvate Sulfurtransferase (MST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 3-Mercaptopyruvate Sulfurtransferase (MST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 3-Mercaptopyruvate Sulfurtransferase (MST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 3-Mercaptopyruvate Sulfurtransferase (MST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該兔SMAD家族成員3(SMAD3)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
