This assay has high sensitivity and excellent specificity for detection of Natural Cytotoxicity Triggering Receptor 3 (NCR3). No significant cross-reactivity or interference between Natural Cytotoxicity Triggering Receptor 3 (NCR3) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Activin A Receptor Type II A (ACVR2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Activin A Receptor Type II A (ACVR2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Activin A Receptor Type II A (ACVR2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Activin A Receptor Type II A (ACVR2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人天然細胞毒性觸發受體3(NCR3)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
