This assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 3 Alpha (HIF3a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 3 Alpha (HIF3a) and analogues was observed.
產品描述
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Neurofilament, Light Polypeptide (NEFL) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Neurofilament, Light Polypeptide (NEFL) and unlabeled Neurofilament, Light Polypeptide (NEFL) (Standards or samples) with the pre-coated antibody specific to Neurofilament, Light Polypeptide (NEFL). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Neurofilament, Light Polypeptide (NEFL) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Neurofilament, Light Polypeptide (NEFL) in the sample.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人缺氧誘導因子3Alpha(HIF3a)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
