This assay has high sensitivity and excellent specificity for detection of Neurofibromin 1 (NF1). No significant cross-reactivity or interference between Neurofibromin 1 (NF1) and analogues was observed.
產品描述
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arachidonate Lipoxygenase 3 (ALOXE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arachidonate Lipoxygenase 3 (ALOXE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arachidonate Lipoxygenase 3 (ALOXE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arachidonate Lipoxygenase 3 (ALOXE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
產品特點
Double-antibody Sandwich
保存建議
Biomatik品牌廠家建議該人神經纖維蛋白1(NF1)ELISA試劑盒產品，Short term: 4°C; Long term: see manual.
