- 中文名稱
Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay
- 英文名字
- Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay
- 供應商
- USBiological
- 產品貨號
- I7506-05
- 產品報價
- ￥1/50 Slides
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- USBiological品牌是全球有名的抗原抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理，是行業中少有的致力于服務客戶，幫助客戶，且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應，專業的產品和解決方案支持，穩健快捷的交貨周期，優質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！
- 產品描述
- The Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay kit is designed to allow immunohistochemical studies of mouse or rat samples using mouse, rabbit, rat and guinea pig primary antibodies. The kit can be used with all common sample preparation methods including: frozen tissues, paraffin-embedded sections, and cell smears. Unlike most immunostaining systems, in which the anti-mouse secondary antibody causes non-specific staining of endogenous rodent immunoglobulins, the kit uses the a specific method to prevent this unwanted staining. The kit incorporates with a high sensitive horseradish peroxidase (HRP) second antibody to increase sensitivity. And the detection system is faster and cleaner because it uses one step of HRP conjugated to second antibody instead of two steps, as in the LAB-SA method.Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay does not contain biotin or streptavidin, so potential background due to endogenous biotin activity is completely avoided. In addition, a special blocking system is used to eliminate background staining. After a user-supplied primary antibody is incubated with the sample, a HRP-conjugated, affinity-purified secondary antibody is bound to the primary antibody. A DAB chromogen/substrate system then creates an intense brown deposit around the antigen/antibody/enzyme complex in the sample.The kit uses a horseradish peroxidase-conjugated second antibody, and a substrate-chromogen mixture to demonstrate antigen in cells or tissues.The principle of this staining system is as follows:1) Paraffin embedded tissue should be deparaffinized and rehydrated.Endogenous peroxidase activity can be quenched by treating tissue sections with 3% hydrogen peroxide in absolute methanol.2) Non-specific background is eliminated by incubating the tissue section with blocking solution A (I7506-05A), and blocking solution B (I7506-05B).3) The mouse, rabbit, rat or guinea pig primary antibody, for a specific antigen, is incubated on the tissue.4) Incubation is followed by addition of a HRP-conjugated second antibody (I7506-05C), binding to the primary antibody.5) The presence of peroxidase can be revealed by addition of substrate chromogen solution (I7506-05D, I7506-05E & I7506-05F: see protocol).Peroxidase will catalyze the substrate (hydrogen peroxide) and convert the chromogen (DAB) to a brown deposit, which demostrates the location of the antigen.The kit is designed to reveal antigens on mouse or rat tissue or cell samples that have reacted with a user-supplied, primary antibody. Depending on the reactivity of the primary antibody, antigens such as cell surface markers, hormones, cytoskeletal proteins and tumor markers can be detected on properly prepared samples. Frozen or paraffin-embedded tissues, freshly prepared lymphocytes, and fixed cultured cells are the most common preparations. Use for research only.Kit Components:I7506-05A: Blocking Solution A, 1x6ml (ready-to-use).I7506-05B: Blocking Solution B, 1x6ml (ready-to-use).I7506-05C: Secondary Antibody (HRP), 6ml (ready-to-use).I7506-05D: Substrate Buffer (20X), 1x2mlI7506-05E: DAB Chromogen (20X), 1x2mlI7506-05F: 0.6% H2O2 (20X), 1x2mlI7506-05G: Hematoxylin Solution, 1x6ml (ready-to-use)I7506-05H: Mounting Solution, 1x10ml (ready-to-use)Storage and Stability:May be stored at 4°C. Do not freeze. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Immunohistochemistry Detection Kit, HRP, Mouse, Rat, BioAssay產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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