- 中文名稱
膠原酶（膠原降解/ Zymography）分析試劑盒（熒光法）
- 英文名字
- Collagenase (Collagen Degradation/Zymography) Assay Kit (Fluorometric)
- 供應商
- Biovision
- 產品貨號
- K490-100
- 產品報價
- ￥詢價/100assays
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868.
- 產品新聞
- 背景資料
- 待更新
- 產品描述
- Collagenases, members of the matrix metalloproteinase (MMP) family, are?endopeptidases that digest native collagen in the triple helix region and are involved in the physiological and pathological turnover of connective tissues. Collagens are the major fibrous component of animal extracellular connective tissue. Bacterial collagenases differ from vertebrate collagenases in that they exhibit broader substrate specificity. Collagenases may play a role in facilitating tumor cell invasion of the extracellular matrix at multiple stages of the metastatic process.?Collagenases have also been approved for medical uses for the treatment of?Dupuytren's contracture, Peyronie's disease and wound healing. Collagenase activity is usually detected by small peptide-based activity assays which may suffer from lack of substrate specificity. Other methods for collagenase activity include collagen Zymography where samples are electrophoresed on a collagen-containing SDS-PAGE, and further renatured in a suitable buffer for 12-16 h. The zymogram is subsequently stained, and areas of digestion appear as clear bands against a darkly stained background where the substrate has been degraded by the enzyme. Such methods are laborious, time-consuming and may lead to the loss of enzymatic activity as renaturation may not be completely reversible. BioVision’s Collagenase Activity Assay Kit utilizes a hybrid approach for the detection of collagenase activity by employing a highly quenched collagen substrate which upon cleavage by a suitable collagenase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of collagenase activity (as low as 0.6 mCDU for bacterial collagenase) in biological samples
- 產品特點
- ? Rapid & sensitive
? Simple assay to measure collagenase activity as well as to screen/study/characterize potential inhibitors of collagenase
? Includes Fluorometric Collagenase Substrate and Positive Control
- 保存建議
- -20
- 其他
- BioVision的公司總部在美麗的舊金山灣地區，它是全球生命科學公司的領導者.BioVision在開發和研究細胞凋亡檢測分析、代謝檢測方面具有卓越的影響力.包括檢測各種檢測分析試劑盒、相關特色抗體、重組蛋白及細胞因子和酶等多種產品，以及其他創新的研究工具.其產品覆蓋細胞凋亡研究、能量代謝、細胞/組織蛋白提取、氧化應激與損傷、代謝與肥胖癥、細胞增殖與毒性、信號轉導、干細胞研究等領域.作為多家知名品牌的中國總代理以及中國區域代理，艾美捷與BioVision一起為研究者們提供最好、最新的細胞生物學研究工具而不斷擴大產品及服務質量.
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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