Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml) - MB-070-010-Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml)

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中文名稱: Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml) - MB-070-010

英文名字: Blocking Buffer for Fluorescent Western Blotting 10-PACK (10 x 500 ml) - MB-070-010

供應商: Rockland

產品貨號: MB-070-010

產品報價: ￥詢價/10x500mL

產品說明書: 點擊查看

購買方式: 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868

產品新聞

背景資料: Western Blot Blocking Buffer is specifically designed for western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. This buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. See www.rockland-inc.com for specific protocols. This product is suitable for use with fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.

產品描述: Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. IRDye?800, IRDye?700DX, Alexa Fluor? 680 and Cy5.5? antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications. IRDye?800 and IRDye?700DX fluoresce outside the range of most autofluorescence and therefore specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. IRDye?800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range. Dual simultaneous labeling in western blots or microscopy is achieved when IRDye?800 conjugates are used in conjunction with IRDye?700 or Cy5.5? conjugates. IRDye?800 and IRDye?700DX conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging. Once reacted with the membrane and dried, IRDye?800 and IRDye?700DX conjugated antibody-protein complexes are very stable, and membranes can be stored protected from light, re-washed and/or rescanned. This blocking buffer is specifically formulated to achieve superior reproducible western blotting images using this system.

產品形式: Liquid (sterile filtered)

保存建議: Store vial at 4° C prior to opening. DO NOT FREEZE.

其他: Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。