BlockOut? (2X) - Universal Blocking Buffer for Western Blotting - MB-073-050-BlockOut? (2X) - Universal Blocking Buffer for Western Blotting

PRODUCT CENTER

生化試劑

產品中心

當前位置：首頁 > 產品中心 > 分子生化產品 > 生化試劑

中文名稱: BlockOut? (2X) - Universal Blocking Buffer for Western Blotting - MB-073-050

英文名字: BlockOut? (2X) - Universal Blocking Buffer for Western Blotting - MB-073-050

背景資料: BlockOut? is an universal blocking buffer solution for Western Blotting. It is specifically designed for colorimetric, chemiluminescent, and fluorescent western blotting. Universal Blocking Buffer is recommended for blocking when phospho specific antibodies are used. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Western Blot blocking buffer is suitable for use with chemiluminiscent and fluorescent western blot imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.

產品描述: BlockOut? is a 2X concentrated stock solution. Prepare a 1X working solution by diluting 1 part 2X concentrate with 1 parts TBS or equivalent. This product allows for superior signal detection and lower background noise in colorimetric, chemiluminescent, and fluorescent western blotting and other applications making it a convenient general purpose blocking agent. Antibody conjugates prepared with IRDye? 800 and IRDye? 700DX (Licor), Cy2?, Cy3?, Cy3.5?, Cy5? and Cy5.5? (GE Healthcare), DyLight?405, DyLight? 549, DyLight? 649, DyLight? 680, and DyLight? 800 (Thermo Fisher/Pierce) and Alexa Fluor? 488, Alexa Fluor? 532, Alexa Fluor? 546, Alexa Fluor? 647 and Alexa Fluor? 680 (Invitrogen/Molecular Probes) and ATTO (Atto-Tec) have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with BlockOut? can be dried and are very stable.

其他: Rockland成立于1962年，是一家老牌抗體原產企業，其位于美國費城郊區的農場保有近20種實驗動物。Rockland公司為生命科學領域提供豐富的、高質量的抗體，包括載脂蛋白、細胞周期/凋亡、細胞因子、細胞信號蛋白、酶類、細胞外基質、轉錄因子(NFkB)和泛素（Ubiquitin）等識別抗體，應用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標記二抗，應用十分廣泛。