Full service includes the generation and production of a target specific single-domain antibody (sdAb) from a camelid VhH library. The sdAb is generated from a random yeast display library composed of VhH genes. Using a specialized selection procedure, individual yeast cells expressing target specific sdAb are isolated. The isolated VhH genes are subcloned and the recombinant sdAb proteins are expressed and purified from E.coli. Each recombinant VhH sdAb can be characterized and qualified for functionality in the specific cell-based assay of interest which can include western blot, immunoprecipication, immunofluorescence, immunohistochemistry, and for platform-specific high-throughput screening.
產品描述
Project phases: nPhase I: Preparation of yeast surface display camelid single domain antibody library and related materials for nanobody Isolation by FACSnnPhase II: Isolation of Yeast Antibody Clones by Fluorescence-Activation Cell Sorting (FACS) and re-cloned into E. coli vectorsnnPhase III: Purified VHH Nanobodies and Characterization for Binding Activity to Native Antigen in Cell-Based Assays
產品形式
該MOLECULAR BIOLOGY SERVICE - Single domain VHH antibody (SdAb) development - MBS-050的詳細信息查看Rockland提供的產品說明書
保存建議
該MOLECULAR BIOLOGY SERVICE - Single domain VHH antibody (SdAb) development - MBS-050的詳細信息查看Rockland提供的產品說明書
