Measure real-time cAMP signaling kinetics in the nucleus with this live cell fluorescent assay. Add NLS-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, red fluorescent membrane-targeted cAMP sensors, or red fluorescent sensors for DAG or Calcium can easily be co-expressed with this assay.
產品描述
用這種活細胞熒光測定法測量細胞核中的實時cAMP信號傳導動力學。將NLS標記的cADD添加到您的細胞中，并在第二天在熒光成像系統上測量強大的信號。減少的綠色熒光指示cAMP增加。該檢測試劑在BacMam中進行遺傳編碼和包裝，可在大多數細胞類型中進行有效轉導-包括在HEK 293細胞中的均勻和接近100%的效率以及在許多原代或iPSC衍生細胞中的表達。該試劑盒包括cAMP傳感器BacMam、500 mM丁酸鈉水溶液以及陽性對照受體和激動劑（B2 AR BacMam和10 mM異丙腎上腺素HCl溶液）。30 mL大小也是可用的。其他Montana分子工具，包括BacMam包裝的GPCR、紅色熒光膜靶向cAMP傳感器或DAG或鈣的紅色熒光傳感器，可以很容易地與該測定共表達。
