Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing green fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs can easily be co-expressed with this assay.
產品描述
測量二酰基甘油（DAG）信號動力學，篩選Gq活性的GPCR，以及更多的活細胞熒光檢測。將DAG傳感器添加到您的細胞中，并在第二天在自動酶標儀或熒光成像系統上測量穩健的信號。增加的綠色熒光指示DAG增加。這些檢測試劑在BacMam中進行基因編碼和包裝，可在大多數細胞類型中進行有效轉導-包括在HEK 293細胞中的均勻和接近100%的效率，以及在許多原代或iPSC衍生細胞中的表達。該試劑盒包括DAG傳感器BacMam、500 mM丁酸鈉水溶液以及陽性對照受體和激動劑（hM 1 R BacMam和25 mM卡巴膽堿水溶液）。30 mL和60 mL的尺寸也可用。其他Montana Molecular工具，包括BacMam包裝的GPCR，可以很容易地與該測定共表達。
