- 中文名稱
DAPI染色液，IHC相關試劑，用于免疫熒光實驗中的細胞核復染
- 英文名字
- DAPI Stain Solution, IHC Related Reagent, For nucleus restaining in Immunofluorescence experiments
- 供應商
- Nordic BioSite
- 產品貨號
- NDC-KBB-GFQWRU-10
- 產品報價
- ￥詢價/10ml
- 產品說明書
- 點擊查看
- 購買方式
- 90%產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- DAPI (4',6-diamidino-2-phenylindole) is a fluorescent dye which can bind DNA strands robustly, the fluorescence being detected by fluorescence microscope. DAPI can dye both live and fixed cells as it can cross intact membrane, with higher efficiency in fixed cells. The molecular formula is C16H15N5 2HCl with 350.25 g/mol molecular weight, CAS Number 28718-90-3. DAPI could pass through the cell and nucleic membranes and bind the double-strand DNA in the nucleus, producing 20 times stronger fluorescence than itself. The efficiency detected by fluorescence microscope is very high (almost 100%), having no side effects for the live cells. The sensitivity for double stranded DNA DAPI staining is many times larger comparing to ethidium bromide (EB). DAPI staining is usually used in cell death detection, as it enters more effectively and generates stronger fluorescence in dead cells. After staining with DAPI, detect with fluorescence microscope or flow cytometry. Blue fluorescent cell would be seen under the microscope after staining. The largest excitation wavelength for DAPI is 340nm (ultraviolet), and the largest emission wavelength is 488nm (blue). When DAPI binds with double-strand DNA, the largest excitation wavelength is 360nm, while the largest emission wavelength becomes 460nm. DAPI's blue emission makes it suitable for combined assays where the fluorescence ranges of DAPI and other IHC-employed fluorescent molecules like green-fluorescent fluorescein and GFP, or red-fluorescent stains like Texas Red, are completely distinctive.
- 產品描述
- DAPI（4 ′，6-二脒基-2-苯基吲哚）是一種能牢固結合DNA鏈的熒光染料，其熒光可通過熒光顯微鏡檢測。DAPI可以染色活細胞和固定細胞，因為它可以穿過完整的膜，在固定細胞中具有更高的效率。分子式為C16 H15 N5 2 HCl，分子量為350.25 g/mol，CAS登記號為28718-90-3。DAPI可以穿過細胞膜和核膜，與細胞核內的雙鏈DNA結合，產生比自身強20倍的熒光。熒光顯微鏡檢測效率很高（幾乎100%），對活細胞無副作用。與溴化乙錠（EB）相比，雙鏈DNA DAPI染色的靈敏度大許多倍。DAPI染色通常用于細胞死亡檢測，因為它更有效地進入并在死細胞中產生更強的熒光。DAPI染色后，熒光顯微鏡或流式細胞儀檢測。染色后顯微鏡下可見藍色熒光細胞。DAPI的最大激發波長為340 nm（紫外），最大發射波長為488 nm（藍光）。當DAPI與雙鏈DNA結合時，最大激發波長為360 nm，而最大發射波長變為460 nm。DAPI的藍色發射使其適合于組合測定，其中DAPI和其他IHC使用的熒光分子（如綠色熒光素和GFP）或紅色熒光染料（如德克薩斯紅）的熒光范圍完全不同。
- 產品特點
- 人CD81（CD81抗原）ELISA試劑盒
- 保存建議
- -20°C
- 其他
- Nordic BioSite(北歐生物) 總部位于瑞典，成立于1997年，旨在分銷研究和診斷領域的高質量和創新產品。 Nordic BioSite 被公認為整個北歐生物醫學研究和診斷產品領域的領導者。他們擁有超過570萬種的產品組合，代理了遍布歐洲和美國的頂級生物試劑制造商，是歐洲知名的一站式生物試劑代理商。除了作為分銷商之外，Nordic BioSite 還擁有不斷增長的自產產品線——BioSite mAb、BioSite ELISA、BioSite Flow、Optibodies和 BioSite Protein, 目前艾美捷代理的是Nordic BioSite自產產品線(共26W+產品)。Nordic BioSite的產品類型主要是抗體，蛋白和試劑盒，產品適用范圍涵蓋15個不同的物種(人，牛，犬，鯰魚，雞，海豚，馬，貓，豚鼠，小鼠，綿羊，兔子，大鼠，豬和火雞)，可為您提供一站式的生命科學研究解決方案~
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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