- 中文名稱
唾液外泌體純化試劑盒（提取試劑盒）
- 英文名字
- Saliva Exosome Purification Kit
- 供應商
- Norgen Biotek
- 產品貨號
- NGB-65300
- 產品報價
- ￥詢價/50Preps
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868。
- 產品新聞
- 背景資料
- 無內容
- 產品描述
- Exosomes are 40 - 150 nm membrane vesicles which are secreted by most cell types. Exosomes can be found in plasma, serum, saliva, urine, amniotic fluid and malignant ascite fluids, among other biological fluids. Evidence has been accumulating recently that these vesicles act as cellular messengers, conveying information to distant cells and tissues within the body. The exosomes contain cell-specific proteins, lipids and RNAs, which are transported to other cells, where they can alter function and/or physiology. These exosomes may play a functional role in mediating adaptive immune responses to infectious agents and tumours, tissue repair, neural communication and transfer of pathogenic proteins. Recent work has demonstrated the presence of distinct subsets of microRNAs within exosomes and other extracellular vesicles (EVs) which depend upon the tumour cell type from which they are secreted. For this reason exosomal RNA may serve as biomarkers for various diseases including cancer. Another subset of RNA that is found in saliva is the free-circulating RNA (fc-RNA). These fc-RNA are usually protein-bound RNA that are leaked from cells either during apoptosis or necrosis. As the RNA molecules encapsulated within exosomes or bound to proteins (fc-RNA) are protected from degradation by RNAses, they can be efficiently recovered from biological fluids such as saliva. In general, these two RNA groups contain valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status.Norgen’s Saliva Exosome Purification Kit allows for the purification of saliva exosomes from 500 μL to 2 mL of fresh saliva samples or saliva samples collected using Norgen's Saliva Exosome Collection and Preservation Kit (Cat 65400). This kit also allows for the purification of intact extracellular vesicles (EVs) from different saliva sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-bound proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.
- 產品特點
- Purification and enrichment of intact saliva exosomes;Versatile sample input ranging from 0.5 mL to 2 mL;Process fresh saliva or saliva collected with Norgen's Saliva;Exosome Collection and Preservation Kit (Cat 65400).;No time-consuming ultracentrifugation, filtration nor special syringes required;No precipitation reagents or overnight incubation required;No protease treatment required;Purify pure exosomes that are free from RNA-binding proteins;Purified exosomes can be analyzed using NanoSight? or Electron Microscopy for assessing the approximate exosome size range and concentration;RNA can be isolated from the purified exosomes using Norgen's Exosome RNA Purification kit (Cat. 58000).
- 保存建議
- 溶液密封室溫(15-25°C)保存，未開封至少保存2年。
- 其他
- Norgen Biotek成立于1998年，公司總部位于加拿大，是一家著名全球化生物技術公司，基于專利的碳化硅膜純化技術和無苯酚專利配方，生產高品質的核酸和蛋白質純化及富集產品用于研究和診斷。Norgen Biotek 在2003年獲得加拿大國家研究委員會頒發的科技創新獎，是一家致力于樣品制備并獲得ISO 9001（產品質量）、ISO 13485（產品可用于商業化的醫療設備，包括用于體外診斷領域）和ISO 15189（可用于臨床檢測機分子診斷領域的研發能力）認證的生物科技公司。
目前該公司的產品多達700余種，提供一流的樣品制備試劑盒，應用于基因組和蛋白組等不同的領域。其產品主要覆蓋包括各種標本類型的DNA，RNA和蛋白質的純化富集，從活檢樣本（包括尿液，血漿，血清和血液）中純化RNA和DNA，全系列的PCR試劑、緩沖液、分子量ladder和塑料過濾裝置。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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