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中文名稱

Pluripotent Stem Cell 3-Color Immunocytochemistry Kit, Human, BioAssay

英文名字
Pluripotent Stem Cell 3-Color Immunocytochemistry Kit, Human, BioAssay
供應商
USBiological
產品貨號
P4285
產品報價
¥1/25Tests
產品說明書
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產品新聞
背景資料
USBiological品牌是全球有名的抗原抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。武漢艾美捷科技作為USBiological品牌中國區域總代理,是行業中少有的致力于服務客戶,幫助客戶,且擁有獨立的專業銷售團隊、技術支持團隊、市場營銷團隊、進出口報關團隊的高科技生物企業。可以為您提供及時的咨詢響應,專業的產品和解決方案支持,穩健快捷的交貨周期,優質放心的售后服務。我們致力于為您提供有價值的產品和服務,在意您的成功!
產品描述
This kit contains three fluorochrome conjugated antibodies that can be used for single-step immunocytochemical staining of human pluripotent stem cells (including embryonic and induced pluripotent stem cells). Each antibody is supplied as 12.5ug in 125ul of PBS containing 0.1% sodium azide.Kit Components:Anti-human SOX2 NL557-Conjugated Goat IgG Anti-human Oct-3/4 NL637-Conjugated Goat IgGAnti-human Nanog NL493-Conjugated Goat IgGIntended Use:This product is designed for the immunocytochemical analysis of human pluripotent stem cells using three fluorochrome-conjugated antibodies.Immunocytochemistry Validation:These antibodies have been tested for immunocytochemistry staining using BG01V cells grown on irradiated mouse embryonic fibroblasts. Cells were fixed in PBS containing 4% paraformaldehyde and blocked with PBS containing 10% normal donkey serum, 0.3% Triton? X-100, and 1% BSA. After blocking, cells were incubated for 3 hours at RT in the dark with all 3 conjugated antibodies at a final concentration of 10ug/ml each (1:10 dilution) in blocking buffer. Between each step, cells were washed with PBS containing BSA. When using a staining volume of 50ul, this kit contains 25 tests.FluorochromeAbsorption Maximum (nm)Emission Maximum (nm)NL557557574NL637637658NL493493514Typical Data:BG01V cells grown on irradiated mouse embryonic fibroblasts were simultaneously stained with (A) NL637- conjugated Oct-3/4, (B) NL493-conjugated Nanog, and (C) NL557-conjugated SOX2. All nuclei were stained with DAPI (shown in panel D).Storage and Stability:Store at 2-8°C in the dark. Use within 6 months of receipt.BG01V cells are licensed from Novocell, Inc. Triton is a registered trademark of Union Carbide.
產品特點
針對Pluripotent Stem Cell 3-Color Immunocytochemistry Kit, Human, BioAssay該產品的特點優勢,歡迎查閱官網提供的產品說明書。
保存建議
建議收到Pluripotent Stem Cell 3-Color Immunocytochemistry Kit, Human, BioAssay產品后將其置于-20°C保存。
其他
Usbiological公司是美國著名的抗體和生化試劑供應商,生產世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品,為科研用戶提供了諸多超值選擇。
注意
該頁面的中文產品信息的翻譯,僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前,請瀏覽說明書確認。
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