WB; IH,Frozen; IH(P), Paraffin Immunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.1-0.5 μg/mL is recommended for WB. Rat NMDAR1 (isoform A) has a predicted length of 938 residues and molecular weight of 106 kDa. A concentration of 0.5-1 μg/mL is recommended to detect NMDAR1 in paraffin embedded tissues. Heat mediated antigen retrieval is required. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
免疫原
A synthetic peptide corresponding to a sequence at the N-terminus of human NMDAR1 (36-53aa RKHEQMFREAVNQANKRH), identical to the related rat and mouse sequences.
來源宿主
兔
反應性
人; 大鼠; predicted to react with 小鼠 due to sequence homology;