- 中文名稱
TUNEL Apoptosis Detection Kit
(DNA Fragmentation/Fluorescence Staining), BioAssay discontinued
- 英文名字
- TUNEL Apoptosis Detection Kit
(DNA Fragmentation/Fluorescence Staining), BioAssay discontinued
- 供應商
- USBiological
- 產品貨號
- T9162-80
- 產品報價
- ￥1/1Kit
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868
- 產品新聞
- 背景資料
- 產品描述
- Suitable for the detection of the endonucleolytic cleavage of chromatin characteristic of apoptosis.Most animal cells can self-destruct via an intrinsic program of cell death that may be triggered by extracellular or intracellular signals. Programmed cell death plays an important role in many biological processes such as morphogenesis and negative selection in the immune system. In addition, dysregulation of programmed cell death may contribute to diseases, for example, cancer, neurodegenerative diseases, and AIDS. Apoptosis is the term for programmed cell death characterized by specific morphologic and biochemical properties. Apoptotic cells typically undergo a series of structural changes: blebbing of the plasma membrane; condensation of the cytoplasm and nuclei; and cellular fragmentation into apoptotic bodies, membrane-bound structures containing condensed cytoplasm, some intact organelles, and nuclear fragments. The most common biochemical property of apoptosis is the endonucleolytic cleavage of chromatin, initially to large fragments of 50-300 kilobase pairs and subsequently to monomers and multimers of 180-200 base pairs. The TUNEL (Terminal deoxynucleotidyl Transferase Biotin-dUTP Nick End Labeling) method identifies apoptotic cells in situ by using terminal deoxynucleotidyl transferase (TdT) to transfer biotin-dUTP to the free 3'-OH of cleaved DNA. The biotin-labeled cleavage sites are then visualized by reaction with fluorescein conjugated avidin (avidin-FITC).Quality Control Testing:Detected cleaved DNA in HeLa cells after incubation with bovine DNase I Capacity: 40 assays using 50ul end-labeling reaction. Kit Components: T9162-80A: Proteinase K (PK), 1x400ugT9162-80B: TdT, (Terminal deoxynucleotidyl Transferase), 1x300u T9162-80C: TdT Buffer, 3x2mlT9162-80D: dUTP (Biotin), 1x100ul*T9162-80E: TB Buffer (10X), 1x50ml*T9162-80F: Blocking Solution, 1x2mlT9162-80G: Avidin (FITC), 1x10ugT9162-80H: Bovine DNase I, 1x200ugStorage and Stability:Store *T9162-80E and T9162-80F at 4°C once thawed. Store other components at -20°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產品特點
- 針對TUNEL Apoptosis Detection Kit
(DNA Fragmentation/Fluorescence Staining), BioAssay discontinued該產品的特點優勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到TUNEL Apoptosis Detection Kit
(DNA Fragmentation/Fluorescence Staining), BioAssay discontinued產品后將其置于4°C/-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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