Hexon protein naturally self assembles as part of the virus life cycle. When the protein is purified we also see evidence of self assembly such as rapid loss of protein upon serial dilution and precipitation. The storage buffer and ELISA coating buffer play an important role in the likelihood of self assembly and thereby can affect results when using purified hexon as an antigen in ELISA and other assays. Many common ELISA coating buffers increase self assembly in hexon protein as they are designed to increase efficiency of antigen adsorption. This results in a very high signal with the initial dilution but then sensitivity tails off very rapidly. Diluting and coating the hexon in water alone slightly increases sensitivity but reduces strength; coating and diluting in the presence of a small amount of stabilizing protein (such as BSA) seems to increase sensitivity for antigen coating significantly (although too much BSA can block high concentration of hexon).
產品形式
Liquid. 10mM Bis Tris Propane, pH 7.2 ,375 mM NaCl, 0.09% Sodium Azide
保存建議
該Adenovirus type 40 hexon protein的保存建議請參考該產品的說明書
其他
Aviva Systems Biology總部位于加利福尼亞州圣迭戈，在中國北京設有辦公室，專注于為研究需求提供多克隆和單克隆抗體、ELISA試劑盒、蛋白質和定制服務。Aviva Systems Biology生產了24,000種經過驗證的多克隆抗體，并提供近20,000種ELISA試劑盒，定制實驗室服務包括蛋白表達和純化、抗體開發，以及ELISA的開發、驗證和生產。Aviva Systems Biology為與獨特物種和靶標相關的研究提供獨特工具，研究領域包括轉錄因子、癌癥、心血管、細胞生物學、DNA損傷和修復、表觀遺傳學、信號轉導、細胞分化、干細胞生物學等等。
