活性氧(ROS)自由基可以直接攻擊生物大分子DNA誘發(fā)DNA氧化損傷。在各種氧化損傷中，鳥苷是DNA核堿基中最易被氧化的堿基，羥基自由基和超氧陰離子會(huì)直接攻擊DNA分子中鳥嘌呤堿基的第8位碳原子，從而生成氧化性加合物8-OhdG（8-羥基脫氧鳥苷）。     

      8-OHdG現(xiàn)已被廣泛地應(yīng)用在實(shí)驗(yàn)研究和臨床診斷中，參與氧化應(yīng)激與病變的指標(biāo)檢測(cè)與機(jī)理分析，對(duì)心血管疾病、糖尿病、神經(jīng)退行性疾病（帕金森病，阿爾茨海默氏癥）、以及癌癥的臨床診療都具有極大的應(yīng)用價(jià)值。目前市場(chǎng)上主流使用的是競爭ELISA方法，因?yàn)樗鼈儾粔蚓_，也不便于應(yīng)用于細(xì)胞/組織的8-OHdG檢測(cè)，更不能直接檢測(cè)從細(xì)胞或組織分離的完整DNA。     為此，艾美捷科技作為專業(yè)的生命科學(xué)領(lǐng)域解決方案供應(yīng)商，為您推薦全球最新的8-OHdG直接定量試劑盒。其檢測(cè)準(zhǔn)確度與HPLC或LC-MS分析高度相關(guān)和接近。   

*8-OHdG試劑盒可直接檢測(cè)提取好的DNA樣品

      8-羥基脫氧鳥苷試劑盒原理

      DNA包被特殊處理帶有高DNA親和性的微孔板上，8-OHdG通過捕獲抗體和檢測(cè)抗體來檢測(cè).通過酶標(biāo)儀可以檢測(cè)結(jié)果.大量得8-OHdG與光密度值成比例.     

<8-OHdG試劑盒實(shí)驗(yàn)流程，標(biāo)準(zhǔn)曲線，以及與液相質(zhì)譜分析對(duì)比>

      8-OHdG試劑盒優(yōu)勢(shì)：

      1、比色實(shí)驗(yàn)簡單易用，方便快速，整個(gè)試驗(yàn)完成需要3小時(shí)45分鐘；

      2、高靈敏度，檢測(cè)8-OHdG極限低至2pg；

      3、高特異性，只能檢測(cè)8-OHdG，不能檢測(cè)8-OHdG類似物；

      4、利用完整DNA直接檢測(cè)8-OHdG，能消除高分子量化合物的影響，例如：糖類和蛋白質(zhì)等能競爭8-OHdG；

      5、檢測(cè)結(jié)果精確，近似接近于HPLC和LC-MS；

      6、高度方便直接使用來自細(xì)胞，組織中的DNA，不需要DNA消化或者水解；

      7、包含通用的陽性對(duì)照和陰性對(duì)照，能適用于定量任何種系8-OHdG；

      8、本試劑盒實(shí)驗(yàn)結(jié)果更精確，靈敏，可靠，可靠并能顯著降低背景信號(hào).

      《8-OHdG試劑盒已發(fā)表文章》

      Smolensky D et. al. (May 2018). High-Polyphenol Sorghum Bran Extract Inhibits Cancer Cell Growth Through ROS Induction, Cell Cycle Arrest, and Apoptosis. J Med Food.

      McLennan D et. al. (April 2018). Telomere elongation during early development is independent of environmental temperatures in Atlantic salmon. J Exp Biol.

      Esplugas R et. al. (February 2018). Renal and hepatic effects following neonatal exposure to low doses of Bisphenol-A and ¹³⁷Cs. Food Chem Toxicol. 114:270-277.

      Ceylan D et. al. (December 2017). DNA redox modulations and global DNA methylation in Bipolar Disorder: Effects of sex, smoking and illness state Psychiatry Research.

      Haque S et. al. (November 2017). Monocyte-derived exosomes upon exposure to cigarette smoke condensate alter their characteristics and show protective effect against cytotoxicity and HIV-1 replication. Sci Rep. 7(1):16120.  Tatsuki       Kunoh et. al. (October 2017). Green Synthesis of Gold Nanoparticles Coupled with Nucleic Acid Oxidation ACS Sustainable Chemistry & Engineering.

      Zhenqiang You et. al. (September 2017). Modulatory Effect of Fermented Papaya Extracts on Mammary Gland Hyperplasia Induced by Estrogen and Progestin in Female Rats Oxidative Medicine and Cellular Longevity. 2017

      Hara K et. al. (September 2017). Scavenging of reactive oxygen species by astaxanthin inhibits epithelial-mesenchymal transition in high glucose-stimulated mesothelial cells. PLoS One. 12(9):e0184332.

      Afshari P et. al. (September 2017). Reduced Slc1a1 expression is associated with neuroinflammation and impaired sensorimotor gating and cognitive performance in mice: Implications for schizophrenia. PLoS One. 12(9):e0183854.

      Tvrda et. al.(July 2017). Antioxidant effects of lycopene on bovine sperm survival and oxidative profile following cryopreservationVeterinarni Medicina. 62(8):429&ndash;436.

      Bell&eacute;s M et. al. (March 2017). Environmental exposure to low-doses of ionizing radiation. Effects on early nephrotoxicity in mice. Environ Res. 156:291-296.

      Noguera JC et. al. (March 2017). Interacting effects of early dietary conditions and reproductive effort on the oxidative costs of reproduction. PeerJ. 5:e3094.

      Ng CT et. al. (February 2017). Zinc oxide nanoparticles exhibit cytotoxicity and genotoxicity through oxidative stress responses in human lung fibroblasts and <i>Drosophila melanogaster</i>. Int J Nanomedicine. 12:1621-1637.

      Tasaki E et. al. (January 2017). An Efficient Antioxidant System in a Long-Lived Termite Queen. PLoS One. 12(1):e0167412.

      Daniel Nettle et. al. (December 2016). Early-life adversity accelerates cellular ageing and affects adult inflammation: Experimental evidence from the European starling Scientific Reports. Dec. 2016

      Szmidt M et. al. (July 2016). Toxicity of different forms of graphene in a chicken embryo model. Environ Sci Pollut Res Int.

      Adamczyk J et. al. (June 2016). Copy number variations of genes involved in stress responses reflect the redox state and DNA damage in brewing yeasts. Cell Stress Chaperones.

      Yost AD et. al. (October 2015). Atmospheric Nonthermal Plasma-Treated PBS Inactivates Escherichia coli by Oxidative DNA Damage. PLoS One. 10(10):e0139903.

      Deregowska A et. al. (October 2015). Genome-wide array-CGH analysis reveals YRF1 gene copy number variation that modulates genetic stability in distillery yeasts. Oncotarget. 6(31):30650-63.

      Philbrook NA et. al. (October 2015). Benzoquinone toxicity is not prevented by sulforaphane in CD-1 mouse fetal liver cells. J Appl Toxicol.

      Shimizu R et. al. (April 2015). Cholangiocyte senescence caused by lysophosphatidylcholine as a potential implication in carcinogenesis. J Hepatobiliary Pancreat Sci.

      Nocchi L et. al. (June 2014). Induction of oxidative stress causes functional alterations in mouse urothelium via a TRPM8-mediated mechanism: implications for aging. Aging Cell. 13(3):540-50.

      Di Nunzio M et. al. (November 2013). Counteraction of oxidative damage by pomegranate juice: influence of the cultivar. J Sci Food Agric. 93(14):3565-73.

      Sobinoff AP et. al. (September 2013). Scrambled and fried: cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress. Toxicol Appl Pharmacol. 271(2):156-67.     

      艾美捷科技與國內(nèi)外優(yōu)秀的生物試劑供應(yīng)商保持著密切的合作關(guān)系，目前已成為眾多國際知名品牌的中國總代理或一級(jí)代理，主要包括：Abbkine、Merck Millipore、Origene、AAT Bioquest、Cayman Chemical、Abnova 、Biovision、ProSpec、Hycult Biotech 、Equitech-Bio、Trevigen、Alomone Labs、Epigentek、ImmunoReagents 、Jackson、SouthernBiotech、US Biological 、Caisson Labs等，可以在第一時(shí)間為用戶提供最前沿的專業(yè)資訊、完備的產(chǎn)品及物流服務(wù)。