使用高N.A.熒光顯微鏡，用綠色cADDis監(jiān)測初級纖毛中cAMP的變化。使用5HT6受體進(jìn)行纖毛定位。表示用于比率測量的紅色熒光標(biāo)簽。還提供了僅針對纖毛的綠色cAMP傳感器。CMV啟動子。

艾美捷 Montana Molecular定量Cilia靶向cADDis cAMP測定試劑盒#D0211G包含的材料：

比率計量的靶向纖毛cADDis cAMP傳感器BacMam

丁酸鈉，500 mM水溶液

用紅色熒光標(biāo)簽靶向纖毛的綠色熒光cAMP傳感器用于比率測量。

Montana Molecular-cAMP部分出版參考：

X. Chen, et al. A PACAP-activated network for secretion requires coordination of Ca2+ influx and Ca2+ mobilization. Molecular Biology of the Cell. May 2024. (bioRxiv)

L. Bridge, et al. Computational modelling of dynamic cAMP responses to GPCR agonists for exploration of GLP-1R ligand effects in pancreatic β-cells and neurons. Cellular Signaling, April 2024.

R. Fagan, et al. Selective targeting of mu opioid receptors to primary cilia. Cell Reports. April 2024.

G. Austin, et al. An inter-organelle contact between endosomal GLP-1R, ER VAP-B, and the mitochondrial AKAP SPHKAP triggers PKA-dependent MIC19 phosphorylation and β-cell mitochondrial remodelling. bioRxiv. April 2024.

I. Cattani-Cavalieri, et al. Real-Time cAMP Dynamics in Live Cells Using the Fluorescent cAMP Difference Detector In Situ. Journal of Visualized Experiments. March 2024.

B. Polacco, et al. Profiling the proximal proteome of the activated μ-opioid receptor. nature chemical biology. March 2024.

P. Balraj, et al. Kisspeptin/KISS1R Signaling Modulates Human Airway Smooth Muscle Cell Migration. American Journal of Respiratory Cell and Molecular Biology. March 2024.

相關(guān)產(chǎn)品：

#D0241G Green Membrane-Targeted fS15 cADDis cAMP

#U0600G Green GECO Ca2+ Assay Kit

#U0600R Red GECO Ca2+ Assay Kit

#U0200G Green Up cADDis cAMP Assay Kit

Montana Molecular產(chǎn)品主要包括GPCR檢測、細(xì)胞應(yīng)激和毒性相關(guān)檢測工具，為客戶提供了長時間內(nèi)觀察候選藥物對細(xì)胞過程影響的能力。艾美捷科技是Montana Molecular的中國代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。