PNA FISH探針因其化學(xué)結(jié)構(gòu)的電中性，能夠與目標(biāo)DNA以高靈敏度和特異性雜交。這一特性使得PNA探針在應(yīng)用到FISH（熒光原位雜交）時更加高效且有用，即使在低濃度下也是如此。雜交發(fā)生得很快（在幾個小時內(nèi)），并且背景噪聲明顯降低。

PNA Bio提供端粒、著絲粒和CAG重復(fù)序列探針作為目錄項目，以及定制PNA探針合成服務(wù)。

TelC是一種富C的端粒探針，用于領(lǐng)先鏈（TAACCC的重復(fù)序列），而TelG是一種富G的端粒探針，用于滯后鏈（TTAGGG的重復(fù)序列）。TelC和TelG PNA端粒探針均可用于人類、小鼠和大鼠染色體。

CENPB探針是著絲粒蛋白B（CENP-B）的結(jié)合位點，CENP-B是主要的著絲粒特異性蛋白之一。探針序列為ATTCGTTGGAAACGGGA，它染色所有人類和小鼠著絲粒的小衛(wèi)星，除了Y染色體。

Cent探針特異性地針對人類α衛(wèi)星著絲粒，不與小鼠發(fā)生反應(yīng)。探針序列為AAACTAGACAGAAGCAT。

艾美捷PNA Bio-PNA FISH探針：

PNA Bio-PNA FISH探針文獻(xiàn)參考：

Integrity of the human centromere DNA repeats is protected by CENP-A, CENP-C, & CENP-T . Giunta S & Funabiki H. (2017) Proc Natl Acad Sci USA 114(8):1928-1933.

Comprehensive characterization of neutrophil genome topology. Zhu Y et al. (2017) Genes & Development. 31(2):141–153.

Long telomeres protect against age-dependent cardiac disease caused by NOTCH1 haploinsufficiency. Theodoris CV et al. (2017) J Clinical Investigation 127(5):1683-1688.

Quantification of telomere features in tumor tissue sections by an automated 3D imaging-based workflow. Gunkel M et al (2017) Methods 114:60-73.

Telomere Replication Stress Induced by POT1 Inactivation Accelerates Tumorigenesis. Pinzaru AM et al. (2016) Cell Rep 15(10):2170-2184.

Regulation of the Human Telomerase Gene TERT by Telomere Position Effect—Over Long Distances (TPE-OLD): Implications for Aging and Cancer. Kim W et al (2016) pLoS Biology 2000016.

Dysfunctional telomeres induce p53‐dependent and independent apoptosis to compromise cellular proliferation and inhibit tumor formation. Wang Y et al (2016) Aging Cell 15(4): 646–660.

ATRX loss promotes tumor growth and impairs nonhomologous end joining DNA repair in glioma. Koschmann C et al (2016) Sci Transl Med. 8(328):328.

Oocyte Polarization Is Coupled to the Chromosomal Bouquet, a Conserved Polarized Nuclear Configuration in Meiosis. Elkouby YM et al. (2016) PLoS Biol. 14(1):e1002335.

Cell Death During Crisis Is Mediated by Mitotic Telomere Deprotection. Hayashi MT et al (2015)Nature 522(7557):492–496.

SMARCAL1 maintains telomere integrity during DNA replication. Poole LA et al. (2015) Proc Natl Acad Sci U S A. 112(48):14864-14869.

艾美捷科技是PNA Bio的中國代理商，為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。