丙酮酸鹽在糖酵解等過程中有重要作用： 它可以在細(xì)胞溶質(zhì)葡萄糖進(jìn)行糖酵解的過程中，由磷酸烯醇式丙酮酸產(chǎn)生。丙酮酸鹽可以繼續(xù)進(jìn)入檸檬酸循環(huán)繼續(xù)氧化分解，轉(zhuǎn)化為碳水化合物或通過乙酰CoA 轉(zhuǎn)化為脂肪酸。高水平的丙酮酸鹽提示肝臟疾病和遺傳性代謝紊亂。丙酮酸鹽也被用來作為減肥刺激劑。在無氧呼吸中丙酮酸可以轉(zhuǎn)化為乳酸或者乙醇。

       作為專業(yè)性的產(chǎn)品供應(yīng)公司，艾美捷科技為您推薦，可同時(shí)用于比色法和熒光法的丙酮酸定量分析試劑盒：K609-100

       * 細(xì)胞或組織樣品需做去蛋白處理（會(huì)消耗丙酮酸），詳情請(qǐng)見說明書
       * 基于客戶儀器設(shè)備，自行選擇比色法或者熒光法即可

       丙酮酸檢測(cè)分析試劑盒原理：

       丙酮酸鹽檢測(cè)試劑盒提供了一種簡(jiǎn)單、直接和可自動(dòng)化操作的程序來測(cè)量不同生物學(xué)標(biāo)本中丙酮酸的含量。適用標(biāo)本類型包括：食物、細(xì)胞、培養(yǎng)基和發(fā)酵培養(yǎng)基。丙酮酸鹽檢測(cè)試劑盒基本原理如下：丙酮酸鹽被丙酮酸酶氧化，在酶反應(yīng)過程中產(chǎn)生顏色（λ= 570 nm）或者熒光（Ex/Em = 535/587 nm），可使用酶標(biāo)儀或熒光計(jì)檢測(cè)到。光的強(qiáng)度與丙酮酸含量是成正比的，因此通過檢測(cè)光的強(qiáng)度即可精確的得到丙酮酸的含量。試劑盒檢測(cè)范圍為1-10 uM丙酮酸。

《比色法和熒光法標(biāo)準(zhǔn)曲線展示》

       丙酮酸檢測(cè)分析試劑盒發(fā)表文章：

       1.Alenzi, Mohammed et al. (2017) Antiurolithic effect of olive oil in a mouse model of ethylene glycol-induced urolithiasis, Investig Clin Urol. 2017 May;58(3):210-216.
       2.Chem et al., Mitochondrial Citrate Transporter-dependent Metabolic Signature in the 22q11.2 Deletion Syndrome. J. Biol. Chem., Sep 2015; 290: 23240 - 23253.
       3.Chen et al., Cadmium toxicity induces ER stress and apoptosis via impairing energy homoeostasis in cardiomyocytes. Biosci. Rep., Jun 2015; 35: e00214.
       4.Chettimada et al., Hypoxia-induced glucose-6-phosphate dehydrogenase overexpression and -activation in pulmonary artery smooth muscle cells: implication in pulmonary hypertension.Am J Physiol Lung Cell Mol Physiol, Feb 2015; 308: L287 - L300.
       5.Cho, S.C. et. al. DDS, 4,4'-diaminodiphenylsulfone, extends organismic lifespan. PNAS, Nov 2010; 107: 19326 - 19331.
       6.Luitpold Fried et al., Identification of a Target Gene and Activating Stimulus for the YpdA/YpdB Histidine Kinase/Response Regulator System in Escherichia coli. J. Bacteriol., Feb 2013; 195: 807 - 815.
       7.McNeely, K. et al. Redirecting Reductant Flux into Hydrogen Production via Metabolic Engineering of Fermentative Carbon Metabolism in a Cyanobacterium. Appl. Envir. Microbiol., 2010; 10.1128/AEM.00862-10
       8.Min You et al., Ethanol administration exacerbates the abnormalities in hepatic lipid oxidation in genetically obese mice. Am J Physiol Gastrointest Liver Physiol, Jan 2013; 304: G38 - G47
       9.Müller et al., Engineering of Ralstonia eutropha H16 for Autotrophic and Heterotrophic Production of Methyl Ketones. Appl. Envir. Microbiol., Jul 2013; 79: 4433 - 4439.
       10.Negi et al., A Carbon-Nitrogen Lyase from Leucaena leucocephala Catalyzes the First Step of Mimosine Degradation. Plant Physiology, Feb 2014; 164: 922 - 934.
       11.Odet, F. et. al. Lactate Dehydrogenase C and Energy Metabolism in Mouse Sperm. Biol Reprod, Sep 2011; 85: 556 - 564.
       12.Palamiuc et al., A metabolic switch toward lipid use in glycolytic muscle is an early pathologic event in a mouse model of amyotrophic lateral sclerosis. EMBO Mol Med., May 2015; 7: 526 - 546.
13.Park et al., Changes in Pyruvate Metabolism Detected by Magnetic Resonance Imaging Are Linked to DNA Damage and Serve as a Sensor of Temozolomide Response in Glioblastoma Cells.Cancer Res., Dec 2014; 74: 7115 - 7124.

       艾美捷科技與國內(nèi)外優(yōu)秀的生物試劑供應(yīng)商保持著密切的合作關(guān)系，目前已成為眾多國際知名品牌的中國總代理或一級(jí)代理，主要包括：Abbkine、Merck Millipore、Origene、AAT Bioquest、Cayman Chemical、Abnova 、Biovision、ProSpec、Hycult Biotech 、Equitech-Bio、Trevigen、Alomone Labs、Epigentek、ImmunoReagents 、Jackson、SouthernBiotech、US Biological 、Caisson Labs等，可以在第一時(shí)間為用戶提供最前沿的專業(yè)資訊、完備的產(chǎn)品及物流服務(wù)。