SiR-微管蛋白是基于微管結(jié)合藥物多西他賽的。SiR-微管蛋白具有熒光特性、細(xì)胞通透性，并且對(duì)微管具有很高的特異性。SiR-微管蛋白能夠在不需要基因操作或過(guò)表達(dá)的情況下對(duì)內(nèi)源性微管進(jìn)行染色。其遠(yuǎn)紅光發(fā)射最大限度地減少了光毒性以及樣本的自發(fā)熒光。SiR-微管蛋白與綠色熒光蛋白（GFP）和/或 m-櫻桃熒光蛋白兼容。可以使用標(biāo)準(zhǔn)的 Cy5 濾光片組進(jìn)行成像。SiR-微管蛋白可用于活細(xì)胞和組織的寬場(chǎng)、共聚焦、SIM 或 STED 成像。探針數(shù)量允許進(jìn)行 50 - 200 次染色實(shí)驗(yàn)。

作為Spirochrome在中國(guó)的區(qū)域總代理，艾美捷科技強(qiáng)烈推薦Spirochrome熱銷產(chǎn)品SiR-微管蛋白試劑盒。產(chǎn)品僅用于科研，不可用于臨床診斷。

SiR-微管蛋白試劑盒

光學(xué)性質(zhì)

λabs 652 nm

εmax 1.0×10⁵ mol^-1·cm^-1

λfl 674 nm

Spirochrome的新產(chǎn)品 SPY650-微管蛋白是 SiR-微管蛋白的改進(jìn)版本。SPY650-微管蛋白在染色細(xì)胞時(shí)能夠發(fā)出更亮的信號(hào)，并且背景更少，同時(shí)仍然兼容超分辨率成像。了解更多關(guān)于 SPY650-微管蛋白的信息，請(qǐng)點(diǎn)擊這里：SPY650-微管蛋白

*基于以下條件：每次染色實(shí)驗(yàn)使用 0.5 - 1 ml 染色液，探針濃度為 0.5 - 1 uM。通過(guò)減少體積或探針濃度，可以進(jìn)一步增加染色實(shí)驗(yàn)的次數(shù)。

最新引用文章

1、Multiscale imaging of cytokinesis

Hümpfer N., Marin Z., ..., Ewers H.

bioRxiv  |  2025 Sep 25  |  Read Article  

"PrePrint: .. cells were seeded onto Geltrex-coated glass-bottom imaging dishes (Zellkontakt, 6160-30).. They were synchronized by a single 2 mM thymidine block for 22 h and released into full medium containing 100 nM SiR-tubulin and 10 μM verapamil (both Spirochrome, SC002) and incubated for 5 h. Prior to live-cell imaging, medium was exchanged to FluoroBrite DMEM (Gibco, A1896701), supplemented with 100 nM SiR-tubulin and 10 μM verapamil.. Ultrastructure Expansion microscopy (U-ExM) of cytokinetic cells.Ultrastructure Expansion microscopy (U-ExM) .." More...

2、Spatiotemporal Analysis of Glucagon Secretory Granule Dynamics.

Samuele Ghignoli, Valentina De Lorenzi, ..., Francesco Cardarelli

Traffic (Copenhagen, Denmark)  |  2025 Sep 24  |  40988617  |  Read Article  

".. upkeeping medium and after 15 min of incubation, unless otherwise specified, imaging was performed.. For assessing cytoskeletal components depolymerization, cells were treated for 30 min with 10 μM SiR- Tubulin (Spirochrome, cat# SC002 [62]) to label microtubules or with 10 μM SiR- Actin (Spirochrome, cat# SC001 [62]) to label actin filaments.. For these treatments, 10 μM Nocodazole (Sigma- Aldrich, cat# 487928) and 10 μM Latrunculin- .." More...

3、Accessibility of the unstructured α-tubulin C-terminal tail is controlled by microtubule lattice conformation

Hotta Takashi, Pimm Morgan L., ..., Verhey Kristen J.

bioRxiv  |  2025 Sep 23  |  Read Article    |  Bioz AI Brief 

"PrePrint: that lattice state impacts the accessibility of the Y-αCTT. Previous work showed that localization of A1aY1 shifts from cytosolic or plus ends to the microtubule lattice in cultured cells treated with SiR-Tubulin ( Kesarwani et al. 2020 ), a Taxol derivative that can convert GDP-tubulin into a GTP-like expanded state ( Siahaan et al. 2022 ). We thus hypothesized that lattice expansion drives access" More...

Spirochrome 成立于 2015年，總部位于瑞士，是一家專注于活細(xì)胞熒光成像技術(shù)的生物科技公司。公司核心技術(shù)源于國(guó)際權(quán)威學(xué)術(shù)期刊《Nature Chemistry》與《Nature Methods》發(fā)表的兩項(xiàng)創(chuàng)新研究成果—— SIR與SPY熒光母核。該類染料具有卓越的穩(wěn)定性、超高熒光信號(hào)與極低背景噪聲，能夠突破傳統(tǒng)成像限制，適用于多種超分辨熒光成像技術(shù)， 助力科學(xué)家開(kāi)啟活細(xì)胞微觀世界的分子尺度探索時(shí)代。

作為Spirochrome在中國(guó)的區(qū)域總代理，艾美捷科技有限公司將為中國(guó)客戶提供全面的Spirochrome產(chǎn)品以及客戶訂制化服務(wù)，歡迎大家隨時(shí)聯(lián)系我們。