晚期氧化蛋白產(chǎn)物（AOPP，Advanced Oxidation Protein Products）是血漿蛋白被體內(nèi)活性氧（ROS）攻擊后形成的氧化修飾產(chǎn)物，是新近發(fā)現(xiàn)的一種具有促炎活性氧化應激指標。

1996年Witko-Sarsat等發(fā)現(xiàn)CRF病人血漿中AOPP水平明顯增高，主要存在于白蛋白中，特征是酸性條件下在340nm有特異吸收峰。在體外用次氯酸(HOC1)與白蛋白作用可得到與CRF病人血漿中相似的AOPP。中性粒細胞活化時髓過氧化物酶(MPO)催化產(chǎn)生的HOC1可能修飾血漿蛋白而生成過量AOPP。AOPP在泌尿、呼吸、神經(jīng)結(jié)締組織、婦科和消化等全身多個系統(tǒng)相關(guān)疾病發(fā)生、發(fā)展中的關(guān)鍵作用均已被證實，其在眾多疾病中的發(fā)病機制也逐漸明晰。

作為專業(yè)的生命科學醫(yī)藥原料供應商，Cell Biolabs中國區(qū)金牌代理，艾美捷科技為您推薦：晚期氧化蛋白產(chǎn)物（AOPP）陽性標準品&試劑盒

* 本產(chǎn)品僅適用于科研用途.

FAQ常見問題：

1.試劑盒適用于哪些類型的樣品？

我們的晚期氧化蛋白產(chǎn)物（AOPP）檢測試劑盒可用于檢測高于檢測限以上的任何樣品類型，例如血清，血漿，細胞/組織裂解液（凍存樣品：需在-80℃保存1年以內(nèi)）。

2.應該如何準備裂解液樣本？

我們建議在含有蛋白酶抑制劑的1XPBS中通過超聲或勻漿裂解細胞或組織，以12000g離心10分鐘，然后收集上清液作為裂解液。我們只推薦使用PBS制備裂解液，因為我們不知道裂解緩沖液是否會損壞AOPP。蛋白質(zhì)濃度應通過蛋白質(zhì)測定法確定，例如BCA或Bradford。蛋白質(zhì)濃度將取決于樣品，但建議在運行實驗之前進行樣品滴定以確定任何必要的稀釋度，以便樣品落在標準曲線內(nèi)。

3.晚期氧化蛋白產(chǎn)物（AOPP）濃度如何展示結(jié)果？

樣品 AOPP 的濃度以氯胺單位 (uM) 表示 (可參考下方發(fā)表文獻)。

結(jié)果展示：

發(fā)表文章：

• Nukala, S.B. et al. (2021). Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension. Sci Rep. 11(1):5583. doi: 10.1038/s41598-021-85004-z (#STA-318).

• AlMarabeh, S. et al. (2020). Chronic intermittent hypoxia impairs diuretic and natriuretic responses to volume expansion in rats with preserved low-pressure baroreflex control of the kidney. Am J Physiol Renal Physiol. doi: 10.1152/ajprenal.00377.2020 (#STA-318).

• Xiao, L.L. et al. (2020). Using advanced oxidation protein products and ischaemia-modified albumin to monitor oxidative stress levels in patients with drug-induced liver injury. Sci Rep. 10(1):18128. doi: 10.1038/s41598-020-75141-2 (STA-318).

• Bloomer, R.J. et al. (2020). Meta- bolic Health Outcomes Following Nine Months of Mild Caloric Restriction in Male Rats Adhering To a Western or Vegan Diet. J Altern Complement Integr Med. 6:097. doi: 10.24966/ACIM-7562/100097 (#STA-318).

• Bigazzi, R. et al. (2019). Hypertension in High School Students: Genetic and Environmental Factors: The HYGEF Study. Hypertension. 75(1):71-78. doi: 10.1161/HYPERTENSIONAHA.119.13818 (#STA-318).

• McAllister, M.J. et al. (2019). Acute coffee ingestion with and without medium chain triglycerides decreases blood oxidative stress markers and increases ketone levels. Can J Physiol Pharmacol. doi: 10.1139/cjpp-2019-0458 (#STA-318).

• Ungurianu, A. et al. (2019). Preclinical and clinical results regarding the effects of a plant-based antidiabetic formulation versus well established antidiabetic molecules. Pharmacol Res. 150:104522. doi: 10.1016/j.phrs.2019.104522 (#STA-318).

• Smith, C. et al. (2019). Chronic testosterone deprivation sensitizes the middle-aged rat brain to damaging effects of testosterone replacement. Neuroendocrinology. doi: 10.1159/000504445 (#STA-318).

• Gryszczyńska, B. et al. (2019). Advanced Oxidation Protein Products and Carbonylated Proteins Levels in Endovascular and Open Repair of an Abdominal Aortic Aneurysm: The Effect of Pre-, Intra-, and Postoperative Treatment. BioMed Research International. 2019(7976043)1-9 pages. doi: 10.1155/2019/7976043 (#STA-318).

• Morsy, M.D. et al. (2019). Protective effect of combined melatonin and α-tocopherol administration in spinal cord ischemia-reperfusion injury in rat. Int. J. Morphol. 37(2):428-437. doi: 10.4067/S0717-95022019000200428 (#STA-318).

• Albatayneh, E.M. et al. (2019). Serum Oxidative-Antioxidative Status in Patients With Alkaptonuria. J Clin Med Res. 11(5):337-344. doi: 10.14740/jocmr3801 (#STA-318).

• Shell, B. et al. (2019). Angiotensin Type 1a Receptors in the Median Preoptic Nucleus Support Intermittent Hypoxia-Induced Hypertension. Am J Physiol Regul Integr Comp Physiol. doi: 10.1152/ajpregu.00393.2018 (#STA-318).

• Bloomer, R. et al. (2018) Chronic Marijuana Smoking Does Not Negatively Impact Select Blood Oxidative Stress Biomarkers in Young, Physically Active Men and Women. Health. 10:960-970. doi: 10.4236/health.2018.107071 (#STA-318).

• Sighinolfi, G. et al. (2018). AB0760 Advanced oxidation protein products in serum of patients with systemic sclerosis: a possible indicator of clinical evolution. Annals of the Rheumatic Diseases. 77:1516. doi: 10.1136/annrheumdis-2018-eular.6809 (#STA-318).

• Wilson, E.N. et al. (2018). Chronic intermittent hypoxia induces hormonal and male sexual behavioral changes: Hypoxia as an advancer of aging. Physiol Behav. 189:64-73. doi: 10.1016/j.physbeh.2018.03.007 (#STA-318).

• Gradinaru, D. et al. (2018). Insulin-Leptin Axis, Cardiometabolic Risk and Oxidative Stress in Elderly with Metabolic Syndrome. Exp Clin Endocrinol Diabetes. doi: 10.1055/s-0043-123825 (#STA-318).

• Hány?ová, S. et al. (2017). Elevated plasma levels of advanced oxidation protein products in Slovak multiple sclerosis patients: possible association with different disability states. Act Nerv Super Rediviva. 59(2): 45–50 (#STA-318).

• Sun, S. et al. (2018). Advanced oxidation protein products induce S-phase arrest of hepatocytes via the ROS-dependent, β-catenin-CDK2-mediated pathway. Redox Biol. 14:338-353. doi: 10.1016/j.redox.2017.09.011 (#STA-318).

• Snyder, B. et al. (2017). Chronic intermittent hypoxia induces oxidative stress and inflammation in brain regions associated with early-stage neurodegeneration. Physiol. Rep. doi: 10.14814/phy2.13258 (#STA-318).

• Budzyń, M. et al. (2017). The Association of Serum Thrombomodulin with Endothelial Injuring Factors in Abdominal Aortic Aneurysm. Hindawi BioMed Research International. doi: 10.1155/2017/2791082 (#STA-318).

• Wan, X. et al. (2016). SIRT1-PGC1a-NFkB pathway of oxidative and inflammatory stress during Trypanosoma cruzi infection: benefits of SIRT1-targeted therapy in improving heart function in Chagas disease. PLoS Pathog. doi: 10.1371/journal.ppat.1005954 (#STA-318).

• Gradinaru, D. et al. (2016). Adiponectin: possible link between metabolic stress and oxidative stress in the elderly. Aging Clin Exp Res. doi:10.1007/s40520-016-0629-z (#STA-318).

• Crone, L. B. et al. (2016). Impact of meal ingestion rate and caffeine coingestion on postprandial lipemia and oxidative stress following high-fat meal consumption. J Caffeine Res. doi:10.1089/jcr.2016.0004 (#STA-318).

• Huemer, M. et al. (2016). Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency. J Inherit Metab Dis. doi:10.1007/s10545-016-9928-y (#STA-318).

• Martins, L. S. et al. (2015). Advanced Glycation Endproducts (AGE) evolution after pancreas-kidney transplantation: plasmatic and cutaneous assessments. Oxid Med Cell Longev. 2189582 (#STA-318).

• ?ari?, B. et al. (2015). Oxidative stress impact on growth hormone secretion in the eye. Croat Med J. 56:326-333 (#STA-318).

• Bloomer, R. J. et al. (2015). Comparison of a restricted and unrestricted vegan diet plan with a restricted omnivorous diet plan on health-specific measures. Healthcare.3:544-555 (#STA-318).

• Jung, E. et al. (2015). Gemigliptin improves renal function and attenuates podocyte injury in mice with diabetic nephropathy. Eur J Pharmacol. doi: 10.1016/j.ejphar.2015.04.055 (#STA-318).

• Thurmond, P. et al. (2015). Structural modifications of the prostate in hypoxia, oxidative stress, and chronic ischemia. Korean J Urol. 56:187-196 (#STA-318)..

• Xie, Z. X. et al. (2014). Effect of GABA on oxidative stress in the skeletal muscles and plasma free amino acids in mice fed high-fat diet. J Anim Physiol Anim Nutr (Berl). doi: 10.1111/jpn.12254 (#STA-318).

【傳送門】AGE-BSA—最好的晚期糖基化終末產(chǎn)物（AGEs）標準品 yyds

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