5-羥甲基胞嘧啶（5-hydroxymethylcytosine，5-HT）作為第六種DNA堿基，在人類和小鼠的腦細胞和胚胎干細胞中含量豐富，具有轉(zhuǎn)錄調(diào)控功能。在哺乳動物中，它可以通過5-甲基胞嘧啶的氧化產(chǎn)生，這是由胞嘧啶加氧酶的10 - 11易位（TET）家族介導(dǎo)的反應(yīng)。

胞嘧啶加氧酶的TET家族包括TET 1、TET 2和TET 3。這些泰特蛋白可以通過結(jié)合到富含CpG的區(qū)域以防止不需要的DNA甲基轉(zhuǎn)移酶活性，以及通過將5 mC轉(zhuǎn)化為5 hmC并通過羥化酶活性進一步轉(zhuǎn)化為5caC（5-羧基胞嘧啶）來促進DNA去甲基化。結(jié)果表明，基因組5 hmC水平與泰特羥化酶活性相關(guān)。此外，TET 1通過結(jié)合ES細胞中的不同靶基因而顯示出在轉(zhuǎn)錄激活和抑制中具有雙重功能。TET 1也是急性髓性白血病中MLL基因的融合伴侶，被認為是一種癌蛋白。發(fā)現(xiàn)TET 2在白血病中經(jīng)常突變，并被認為是腫瘤抑制劑。TET 3被證明在哺乳動物受精卵中的DNA甲基化重編程過程中發(fā)揮獨特的作用。因此，激活腫瘤抑制泰特酶如TET 2或抑制癌蛋白TET酶如TET 1將在有益于癌癥診斷和開發(fā)新的基于靶點的癌癥治療劑方面是重要的。

那么如何檢測5hmc羥化酶TET的活性呢？

作為專業(yè)的生命科學(xué)解決方案供應(yīng)商，艾美捷為您推薦Nature同款方案：

5hmC-羥化酶TET活性/抑制分析試劑盒 優(yōu)點和特點：

1、5小時比色測定程序，步驟簡單，方便快捷。整個過程可在5小時內(nèi)完成。

2、通過直接檢測TET轉(zhuǎn)化的羥甲基化產(chǎn)物直接測量泰特羥化酶活性。

3、創(chuàng)新的試劑盒組成使背景信號極低，并允許檢測簡單、準確、可靠和一致。

4、可以使用細胞/組織提取物或純化泰特蛋白，其允許檢測TET羥化酶抑制劑在體內(nèi)和體外的抑制作用。

5、新的測定原理允許實現(xiàn)高靈敏度。活性可以從低至20ng的純化的TET1羥化酶檢測。

6、包括羥甲基化標準品，其允許定量泰特羥化酶的比活性。 

7、條帶微孔板形式使得該測定方法靈活，可用于手動或高通量分析（96次測定）。

Nature文章結(jié)果：

DOI https://doi.org/10.1038/s41590-020-0764-8

1.TET酶活性分析使用P-3086;  使用艾美捷推薦的P-1032檢測整體5hmc水平

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