單抗分類鑒定首選

在單克隆抗體的制備過程中，雜交瘤細(xì)胞的篩選和鑒別是最重要的步驟。首先需要篩選出正確融合的雜交瘤細(xì)胞，再篩選出能分泌目標(biāo)抗體的雜交瘤細(xì)胞，最終將篩選出來的雜交瘤細(xì)胞進(jìn)行擴(kuò)大培養(yǎng)，制備大量單克隆抗體。在進(jìn)行雜交瘤細(xì)胞的篩選時(shí)，需要鑒定這類雜交瘤細(xì)胞培養(yǎng)上清液中單克隆抗體的類別和亞型，最常用的是ELISA法，因其快速、簡(jiǎn)便、靈敏度高、特異性強(qiáng)、易于標(biāo)準(zhǔn)化等優(yōu)點(diǎn)，已得到廣泛應(yīng)用。

單克隆抗體分型ELISA試劑盒應(yīng)用

單克隆抗體分型ELISA試劑盒運(yùn)用雙抗體夾心法原理，先用Ig捕獲二抗在96孔板上包被固相，然后加入待測(cè)的雜交瘤細(xì)胞培養(yǎng)上清液，其中的目標(biāo)蛋白便會(huì)與96孔板上的固相二抗結(jié)合，洗板去除游離物質(zhì)后加入用于篩選雜交瘤細(xì)胞的標(biāo)記二抗孵育，洗板去除游離物質(zhì)，加入顯色底物顯色，在450nm處測(cè)定OD值，進(jìn)行定性及定量分析。

SBA單克隆抗體分型試劑盒

SouthernBiotech單克隆抗體分型試劑盒專為小鼠單克隆抗體的分型設(shè)計(jì)。分型試劑盒包含純化的抗小鼠Ig捕獲抗體和HRP或AP偶聯(lián)的抗小鼠Ig、IgA、IgG1、IgG2a或IgG2c、IgG2b、IgG3、IgM、kappa和lambda抗體，用于檢測(cè)。這些也可用于血清、上清和腹水等樣品中小鼠免疫球蛋白水平的定量研究。

試劑盒有多種形式，可用于檢測(cè)表達(dá)Igh1-a等位基因(如BALB/c)和Igh1-b等位基因(如C57BL/6、C57BL/10、SJL、NOD)的小鼠的免疫球蛋白。使用我們的小鼠免疫球蛋白面板與我們的單克隆抗體分型試劑盒可以生成可靠的標(biāo)準(zhǔn)曲線。

SBA克隆分型系統(tǒng)- HRP試劑盒（貨號(hào)5300-05）含有2.5 mg捕獲抗體和1.0 mL HRP偶聯(lián)抗小鼠Ig、小鼠IgA、小鼠IgG1、小鼠IgG2a、小鼠IgG2b、小鼠IgG3、小鼠IgM、小鼠κ、小鼠λ和ABTS底物。

SBA單克隆抗體分型試劑盒優(yōu)勢(shì)

操作簡(jiǎn)便，結(jié)果準(zhǔn)確，易于分析；

可用于初篩抗體高分泌雜交瘤細(xì)胞株，鑒定陽(yáng)性克隆細(xì)胞的特異性抗體分型(IgA、IgM、IgG1、IgG2a、IgG2b、IgG3), 鑒定抗體kappa、lambda輕鏈異構(gòu)體以及普通抗體分型ELISA研究；

性價(jià)比高，搭配使用可進(jìn)行1000次以上樣本檢測(cè)；

每批試劑盒均經(jīng)過ELISA檢測(cè)以確保批間一致性。

其中5300-01套裝內(nèi)含有小鼠源IgA、IgM、IgG1、IgG2a、IgG2b、IgG3免疫球蛋白標(biāo)準(zhǔn)品，每種均為1ml（濃度為100μg/ml）；而5300-01B套裝內(nèi)用IgG2b替換了5300-01里的IgG2c。而每種SBA單克隆抗體分型試劑盒則包含了1ml親和純化山羊抗小鼠Ig捕獲二抗（用于包被96孔板），1ml酶標(biāo)記山羊抗小鼠Ig二抗（用于初篩雜交瘤細(xì)胞），1ml×8酶標(biāo)記山羊抗小鼠IgA, IgM, IgG1, IgG2a, IgG2b, IgG3, Ig kappa，Ig lambda二抗，酶反應(yīng)底物及操作手冊(cè)；此外還有配套Ig標(biāo)準(zhǔn)品可供選擇。

相關(guān)產(chǎn)品推薦

5300-05 部分引文如下，其他產(chǎn)品的引文請(qǐng)參考官網(wǎng)或聯(lián)系艾美捷：

1. Tseng T, Chung C. A monoclonal antibody recognizing the specific epitope of aflatoxin analogs. Bot Bull Acad Sin. 1990;31:279-84. (ELISA - Isotyping)

2. Yu L, Lee KK, Ens K, Doig PC, Carpenter MR, Staddon W, et al. Partial characterization of a Candida albicans fimbrial adhesin. Infect Immun. 1994;62:2834-42. (ELISA - Isoyping)

3. Esteban MR, Giovinazzo G, de la Hera A, Goday C. PUMA1: a novel protein that associates with the centrosomes, spindle and centromeres in the nematode Parascaris. J Cell Sci. 1998;111:723-35. (ELISA - Isotyping)

4. Gil OD, Zhang L, Chen S, Ren YQ, Pimenta A, Zanazzi G, et al. Complementary expression and heterophilic interactions between IgLON family members neurotrimin and LAMP. J Neurobiol. 2002;51:190-204. (ELISA - Isotyping)

5. Sánchez MD, Pierson TC, McAllister D, Hanna SL, Puffer BA, Valentine LE, et al. Characterization of neutralizing antibodies to West Nile virus. Virology. 2005;336:70-82. (ELISA - Isotyping)

6. Mazzarotto GA, Raboni SM, Stella V, Carstensen S, de Noronha L, Levis S, et al. Production and characterization of monoclonal antibodies against the recombinant nucleoprotein of Araucaria hantavirus. J Virol Methods. 2009;162:96-100. (ELISA - Isotyping)

7. Wang C, deVries S, Camboni M, Glass M, Martin PT. Immunization with the SDPM1 peptide lowers amyloid plaque burden and improves cognitive function in the APPswePSEN1(A246E) transgenic mouse model of Alzheimer's disease. Neurobiol Dis. 2010;39:409-22. (ELISA - Isotyping & Serum)

8. Vassiliadis E, Veidal SS, Simonsen H, Larsen DV, Vainer B, Chen X, et al. Immunological detection of the type V collagen propeptide fragment, PVCP-1230, in connective tissue remodeling associated with liver fibrosis. Biomarkers. 2011;16:426-33. (ELISA - Isotyping)

9. Liu Z, Song C, Li Y, Liu F, Zhang K, Sun Y, et al. Development of highly sensitive chemiluminescence enzyme immunoassay based on the anti-recombinant HC subunit of botulinum neurotoxin type A monoclonal antibodies. Anal Chim Acta. 2012;735:23-30. (ELISA - Isotyping)

10. Han Z, Zhao F, Shao Y, Liu X, Kong X, Song Y, et al. Fine level epitope mapping and conservation analysis of two novel linear B-cell epitopes of the avian infectious bronchitis coronavirus nucleocapsid protein. Virus Res. 2013;171:54-64. (ELISA - Isotyping)

11. Wongwairot S, Kueakhai P, Changklungmoa N, Jaikua W, Sansri V, Meemon K, et al. Monoclonal antibody against recombinant Fasciola gigantica cathepsin L1H could detect juvenile and adult cathepsin Ls of Fasciola gigantica. Parasitol Res. 2015;114:133-40. (ELISA - Isotyping)

12. Ren J, Zhu Y, Zhou Y, Lv C, Yan H, Ma L, et al. Identification of three antigen epitopes on the nucleocapsid protein of the genotype C of bovine parainfluenza virus type 3. Vet Microbiol. 2015;178:61-9. (ELISA - Isotyping)

13. Guo C, Tang Y, Qi Z, Liu Y, Zhao X, Huo X, et al. Development and characterization of a panel of cross-reactive monoclonal antibodies generated using H1N1 influenza virus. Immunobiology. 2015;220:941-6. (ELISA - Isotyping)

14. Li C, Liang W, Liu W, Yang D, Wang H, Ma W, et al. Identification of a conserved linear epitope using a monoclonal antibody against non-structural protein 3B of foot-and-mouth disease virus. Arch Virol. 2016;161:365-75. (ELISA - Isotyping)

15. Gu J, Wang L, Jin Y, Lin C, Wang H, Zhou N, et al. Characterization of specific antigenic epitopes and the nuclear export signal of the Porcine circovirus 2 ORF3 protein. Vet Microbiol. 2016;184:40-50. (ELISA - Isotyping)

16. Hansen NU, Willumsen N, Sand JM, Larsen L, Karsdal MA, Lemming DJ. Type VIII collagen is elevated in diseases associated with angiogenesis and vascular remodeling. Clin Biochem. 2016 May 24. doi: 10.1016/j.clinbiochem.2016.05.023. [Epub ahead of print]. (ELISA - Isotyping)

17. Cascalho M, Wong J, Wabl M. VH gene replacement in hyperselected B cells of the quasimonoclonal mouse. J Immunol. 1997;159:5795-801. (ELISA - Serum)

18. Kikuchi T, Hackett NR, Crystal RG. Cross-strain protection against clinical and laboratory strains of Pseudomonas aeruginosa mediated by dendritic cells genetically modified to express CD40 ligand and pulsed with specific strains of Pseudomonas aeruginosa. Hum Gene Ther. 2001;12:1251-63. (ELISA - Serum)

19. Wu X, Jiang N, Deppong C, Singh J, Dolecki G, Mao D, et al. A role for the Cr2 gene in modifying autoantibody production in systemic lupus erythematosus. J Immunol. 2002;169:1587-92. (ELISA - Serum)

20. Ruzek MC, Hawes M, Pratt B, McPherson J, Ledbetter S, Richards SM, et al. Minimal effects on immune parameters following chronic anti-TGF-β monoclonal antibody administration to normal mice. Immunopharmacol Immunotoxicol. 2003;25:235-57. (ELISA - Serum)

21. Olson TS, Bamias G, Naganuma M, Rivera-Nieves J, Burcin TL, Ross W, et al. Expanded B cell population blocks regulatory T cells and exacerbates ileitis in a murine model of Crohn disease. J Clin Invest. 2004;114:389-98. (ELISA - Serum & Supernatant)

22. Coughlin JJ, Stang SL, Dower NA, Stone JC. RasGRP1 and RasGRP3 regulate B cell proliferation by facilitating B cell receptor-Ras signaling. J Immunol. 2005;175:7179-84. (ELISA - Serum)

23. Perchonock CE, Fernando MC, Quinn WJ 3rd, Nguyen CT, Sun J, Shapiro MJ, et al. Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006;26:6005-15. (ELISA - Serum)

24. Travis MA, Reizis B, Melton AC, Masteller E, Tang Q, Proctor JM, et al. Loss of integrin αvβ8 on dendritic cells causes autoimmunity and colitis in mice. Nature. 2007;449:361-5. (ELISA - Serum)

25. Bell JJ, Divekar RD, Ellis JS, Cascio JA, Haymakker CL, Jain R, et al. In trans T cell tolerance diminishes autoantibody responses and exacerbates experimental allergic encephalomyelitis. J Immunol. 2008;180:1508-16. (ELISA - Serum)

26. Bolduc A, Long E, Stapler D, Cascalho M, Tsubata T, Koni PA, et al. Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J Immunol. 2010;185:220-30. (ELISA - Serum)

27. Bohgaki T, Bohgaki M, Cardoso R, Panier S, Zeegers D, Li L, et al. Genomic instability, defective spermatogenesis, immunodeficiency, and cancer in a mouse model of the RIDDLE syndrome. PLoS Genet. 2011;7(4):e1001381. (ELISA - Serum & Supernatant)

28. Huang C, Hatzi K, Melnick A. Lineage-specific functions of Bcl-6 in immunity and inflammation are mediated by distinct biochemical mechanisms. Nat Immunol. 2013;14:380-8. (ELISA - Serum)

29. Huang Q, Perlman H, Birkett R, Doyle R, Fang D, Haines GK, et al. CD11c-mediated deletion of Flip promotes autoreactivity and inflammatory arthritis. Nat Commun. 2015;6:7086. (ELISA - Serum)

30. Ying Z, Mei M, Zhang P, Liu C, He H, Gao F, et al. Histone arginine methylation by PRMT7 controls germinal center formation via regulating Bcl6 transcription. J Immunol. 2015;195:1538-47. (ELISA - Serum)

31. Benhamron S, Pattanayak SP, Berger M, Tirosh B. mTOR activation promotes plasma cell differentiation and bypasses XBP-1 for immunoglobulin secretion. Mol Cell Biol. 2015;35:153-66. (ELISA - Serum & Supernatant)

32. Zanotti KJ, Maul RW, Castiblanco DP, Yang W, Choi YJ, Fox JT, et al. ATAD5 deficiency decreases B cell division and Igh recombination. J Immunol. 2015;194:35-42. (ELISA - Serum)

33. Chen J, Li Z, Petersen E, Liu W, Zhu X. Co-administration of interleukins 7 and 15 with DNA vaccine improves protective immunity against Toxoplasma gondii. Exp Parasitol. 2016;162:18-23. (ELISA - Serum)

34. Zhao Y, Li Z, Chen J, Sun X, Liu S, Zhu X, ey al. Protective efficacy of pVAX-RON5p against acute and chronic infections of Toxoplasma gondii in BALB/c mice. Exp Parasitol. 2016;163:24-30. (ELISA - Serum)

35. Corcos D, Iglesias A, Dunda O, Bucchini D, Jami J. Allelic exclusion in transgenic mice expressing a heavy chain disease-like human μ protein. Eur J Immunol. 1991;21:2711-6. (ELISA - Supernatant)

36. Ishida K, Yoshimura K. Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis. Parasite Immunol. 1992;14:633-44. (ELISA - Supernatant)

37. Yamada T, Mitani T, Yorita K, Uchida D, Matsushima A, Iwamasa K, et al. Abnormal immune function of hemopoietic cells from alymphoplasia (aly) mice, a natural strain with mutant NF-κB-inducing kinase. J Immunol. 2000;165:804-12. (ELISA - Supernatant)

38. Wittman VP, Woodburn D, Nguyen T, Neethling FA, Wright S, Weidanz JA. Antibody targeting to a class I MHC-peptide epitope promotes tumor cell death. J Immunol. 2006;177:4187-95. (ELISA - Supernatant)

39. Verma B, Hawkins OE, Neethling FA, Caseltine SL, Largo SR, Hildebrand WH, et al. Direct discovery and validation of a peptide/MHC epitope expressed in primary human breast cancer cells using a TCRm monoclonal antibody with profound antitumor properties. Cancer Immunol Immunother. 2010;59:563-73. (ELISA - Supernatant)

40. Zhang L, Zhang H, Fan Z, Zhou X, Yu L, Sun H, et al. Identification of a conserved linear B-cell epitope of Streptococcus dysgalactiae GapC protein by screening phage-displayed random peptide library. PLoS One. 2015;10(6):e0131221. (ELISA - Supernatant & Ascites)

41. Zhang J, Li H, Huo R, Zhai T, Li H, Sun Y, et al. Paeoniflorin selectively inhibits LPS-provoked B-cell function. J Pharmacol Sci. 2015;128:8-16. (ELISA - Supernatant)

42. Zhang L, Zhou X, Fan Z, Tang W, Chen L, Dai J, et al. Identification of a conserved B-cell epitope on the GapC protein of Streptococcus dysgalactiae. Microb Pathog. 2015;83-84:23-8. (ELISA - Supernatant & Ascites)