DNA胞嘧啶的表觀遺傳修飾通過(guò)調(diào)節(jié)基因轉(zhuǎn)錄在發(fā)育中起關(guān)鍵作用。目前研究最多的胞嘧啶修飾是5-甲基胞嘧啶（5mC），它與基因表達(dá)的抑制有關(guān)。在脫甲基化過(guò)程中，5mC氧化為連續(xù)的中間狀態(tài)，包括5-羥甲基胞嘧啶（5hmC），5-甲酰基胞嘧啶（5fC）和5-羧基胞嘧啶（5caC）。在過(guò)去的十年中，研究表明，氧化形式的5mC，尤其是5hmC，除了在活性脫甲基過(guò)程中充當(dāng)“中間體”外，還可能具有多種獨(dú)立的功能。很多研究表明，5hmC 表達(dá)水 平與基因表達(dá)調(diào)控、多能干細(xì)胞分化、神經(jīng)元發(fā)育、以及腫瘤發(fā)生都密切相關(guān)。

而參與其中甲基化循環(huán)的一個(gè)重要酶類(lèi)，就是TET家族蛋白！人TET蛋白家族包含TET1、TET2和TET3這3個(gè)成員，所有TET蛋白都包含一個(gè)保守的雙鏈β-螺旋結(jié)構(gòu)域、一個(gè)富含半胱氨酸區(qū)和C端的核心催化結(jié)構(gòu)域。TET1主要在胚胎干細(xì)胞中表達(dá)，TET2主要在造血系統(tǒng)中表達(dá)，TET3則在 小腦、皮質(zhì)和海馬體等神經(jīng)系統(tǒng)中廣泛表達(dá)。結(jié)果表明，基因組5hmC 水平與 TET 羥化酶活性相關(guān)。

那么如何檢測(cè)5hmc羥化酶TET的活性呢？

作為專(zhuān)業(yè)的生命科學(xué)解決方案供應(yīng)商，艾美捷為您推薦Nature同款方案：

Nature文章結(jié)果：

DOI https://doi.org/10.1038/s41590-020-0764-8

1.TET酶活性分析使用P-3086; c. 使用艾美捷推薦的P-1032檢測(cè)整體5hmc水平

更多客戶(hù)的選擇：

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Wulansari N et. al. (February 2021). LIF Maintains mESC Pluripotency by Modulating TET1 and JMJD2 Activity in a JAK2-Dependent Manner.Stem Cells.

Clark DF et. al. (January 2021). Acute high folic acid treatment in SH-SY5Y cells with and without MTHFR function leads to gene expression changes in epigenetic modifying enzymes, changes in epigenetic marks, and changes in dendritic spine densities.PLoS One. 16(1):e0245005.

Ko?odziej-Wojnar P et. al. (December 2020). Alterations in the Genomic Distribution of 5hmC in In Vivo Aged Human Skin Fibroblasts.Int J Mol Sci. 22(1)

Zhou B et. al. (August 2020). The angiocrine Rspondin3 instructs interstitial macrophage transition via metabolic-epigenetic reprogramming and resolves inflammatory injury.Nat Immunol.

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Gu Y et. al. (July 2020). Hydrogen sulfide attenuates renal fibrosis by inducing TET-dependent DNA demethylation on Klotho promoter.FASEB J.

Aggarwal et. al. (July 2020). Functional succinate dehydrogenase deficiency is a pathognomonic adverse feature of clear cell renal cancer

Sun X. et. al. et. al. (May 2020). AMPK deficiency induces DNA methylation and aggravates colorectal tumorigenesis in 2 AOM/DSS miceResearch Square.

Kaur G et. al. (April 2020). Regulation of DNA methylation signatures on NF-κB and STAT3 pathway genes and TET activity in cigarette smoke extract-challenged cells/COPD exacerbation model in vitro.Cell Biol Toxicol.

Briand J et. al. (April 2020). Radiotherapy-induced overexpression of exosomal miRNA-378a-3p in cancer cells limits natural killer cells cytotoxicity.

Zhou Y et. al. (March 2020). HIF1A activates the transcription of lncRNA RAET1K to modulate hypoxia-induced glycolysis in hepatocellular carcinoma cells via miR-100-5p.Cell Death Dis. 11(3):176.

Bhagat TD et. al. (October 2019). Lactate-mediated epigenetic reprogramming regulates formation of human pancreatic cancer-associated fibroblasts.8

Zhou L et. al. (October 2019). TET2-interacting long noncoding RNA promotes active DNA demethylation of the MMP-9 promoter in diabetic wound healing.Cell Death Dis. 10(11):813.

Parsanathan R et. al. (October 2019). Glutathione deficiency induces epigenetic alterations of vitamin D metabolism genes in the livers of high-fat diet-fed obese mice.Sci Rep. 9(1):14784.

Shi F et. al. (April 2019). EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the <i>RIP3</i> promoter.9(9):2424-2438.

Cramer T et. al. (December 2018). PARP Inhibitor Affects Long-term Heat-stress Response via Changes in DNA Methylation.399:65-76.

Cramer T et. al. (October 2018). Early-life epigenetic changes along the corticotropin-releasing hormone (CRH) gene influence resilience or vulnerability to heat stress later in lifeMolecular Psychiatry.

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Shahid M et. al. (March 2018). Alpha-oxoglutarate inhibits the proliferation of immortalized normal bladder epithelial cells via an epigenetic switch involving ARID1A.Sci Rep. 8(1):4505.

Rea M et. al. (November 2017). Selective inhibition of CTCF binding by iAs directs TET-mediated reprogramming of 5-hydroxymethylation patterns in iAs-transformed cells.Toxicol Appl Pharmacol. 338:124-133.

Wulansari N et. al. (October 2017). Vitamin C-Induced Epigenetic Modifications in Donor NSCs Establish Midbrain Marker Expressions Critical for Cell-Based Therapy in Parkinson's Disease.Stem Cell Reports. 9(4):1192-1206.

Shenoy N et. al. (July 2017). Upregulation of TET activity with ascorbic acid induces epigenetic modulation of lymphoma cells.Blood Cancer J. 7(7):e587.

Prasad R et. al. (May 2017). Honokiol inhibits ultraviolet radiation-induced immunosuppression through inhibition of ultraviolet-induced inflammation and DNA hypermethylation in mouse skin.Sci Rep. 7(1):1657.

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Ishikawa K et. al. (February 2015). Long-term pancreatic beta cell exposure to high levels of glucose but not palmitate induces DNA methylation within the insulin gene promoter and represses transcriptional activity.PLoS One. 10(2):e0115350.

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He XB et. al. (December 2014). Vitamin C Facilitates Dopamine Neuron Differentiation in Fetal Midbrain Through Tet1- aND Jmjd3-Dependent Epigenetic Control Manner.Stem Cells.

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Scola G et. al. (April 2014). Lithium reduces the effects of rotenone-induced complex I dysfunction on DNA methylation and hydroxymethylation in rat cortical primary neurons.Psychopharmacology (Berl).

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Coulter JB et. al. (October 2013). Hydroquinone increases 5-hydroxymethylcytosine formation through ten eleven translocation 1 (TET1) 5-methylcytosine dioxygenase.J Biol Chem. 288(40):28792-800.

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